氟对大鼠切牙成釉细胞内质网伴侣分子表达的影响  

Effect of fluoride on the expression of endoplasmic reticulum chaperone in ameloblast of rat incisor

作　　者：张凯强[1] 张颖[1] 刘璐[1] 顾何锋[1] 马林[1] 

机构地区：[1]中国医科大学口腔医学院口腔预防科,辽宁省口腔医学研究所口腔预防教研室,辽宁沈阳110002

出　　处：《上海口腔医学》2013年第5期481-486,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81072245);辽宁省自然科学基金(20102278)~~

摘　　要：目的:研究不同浓度氟化物对大鼠切牙成釉细胞的影响,探讨内质网应激在氟斑牙形成中的作用。方法:30只Wistar大鼠随机分为3组,建立氟斑牙动物模型。观察大鼠切牙成釉细胞的形态学和GRP78、XBP-1、CRT和caspase-12表达的改变,采用MetaMorph显微图像分析软件和SPSS 13.0软件包对数据进行统计学分析。结果:随着饮水氟离子浓度的升高,切牙逐渐出现氟斑牙症状。免疫组化结果显示,CRT(F=11.72,P<0.05)、GRP78(F=27.42,P<0.05)、XBP-1(F=139.7,P<0.05)、caspase-12(F=43.91,P<0.05)表达随氟离子浓度的升高而升高,且各组间均具有显著性差异。结论:成釉细胞在一定浓度的氟化物作用下,其CRT、GRP78、XBP-1和caspase-12均过表达,表明成釉细胞处于内质网应激状态,且caspase-12是导致细胞凋亡的重要途径。PURPOSE： To investigate the effect of different concentrations of fluoride on the expression of endoplasmic reficulum chaperone, and to explore the mechanism of dental fluorosis in rat. METHODS： Thirty Wistar rats were randomly divided into 3 groups. Immunohistochemistry was used to detect the expression of CRT, GRP78, XBP-1 and caspase-12 in rat incisors. Metamorph microscope images analysis system and SPSS 13.0 software package was used to analyze the data. RESULTS： Typical features of dental fluorosis were found in the fluoride group. Results of immunohistochemistry showed that CRT （F=238.6, P〈0.05）, GRP78 （F=27.42, P〈0.05）, XBP-1 （F=139.7, P〈0.05） and easpase-12 （F=43.91,P〈0.05） were significantly different among the 3 groups. CONCLUSIONS： Excessive fluoride can increase the secretion of CRT, GRP78, XBP-1 and caspase-12 suggest the ameloblasts and in status of endoplasmic reticulum stress and caspase-12 plays an important role during ameloblast apoptosis. Supported by National Natural Science Foundation of China （81072245） and National Science Foundation of Liaoning Province （20102278）.

关 键 词：氟 成釉细胞 内质网应激 

分 类 号：R780.1[医药卫生—口腔医学]