骨形成蛋白信号通路中Smad4和Smad7在口腔鳞癌中的表达变化  

Expression of Smad4 and Smad7 of BMP signaling pathway in oral squamous cell carcinoma

作　　者：刘端芹[1] 张君[1,2] 宋洪宁[1] 郑君[1] 王旭霞[1,2] 

机构地区：[1]山东大学口腔医学院口腔颌面外科,山东济南250012 [2]山东省口腔生物医学重点实验室,山东济南250012

出　　处：《上海口腔医学》2013年第5期492-497,共6页Shanghai Journal of Stomatology

基　　金：山东省科学技术发展项目(2010GSF10239);山东省科学技术专项计划项目(2012G0021852)~~

摘　　要：目的:观察口腔鳞癌和癌旁正常组织中Smad4和Smad7表达量的变化,探讨Smad4和Smad7对口腔鳞癌发生和发展的影响。方法:收集临床和病理学诊断为OSCC的病例72例。取癌组织和癌旁正常组织做切片,用SP免疫组化法分别检测Smad4和Smad7在口腔鳞癌和正常组织中的表达,采用SPSS11.5软件包对数据进行统计学分析。结果:Smad4蛋白在癌旁正常组织中的阳性表达率为69.44%,在口腔鳞癌组织中的阳性表达率为45.83%(P<0.05),且随着分化程度的降低其阳性表达率降低;有淋巴结转移者阳性表达率为22.50%,无淋巴结转移者阳性表达率为65.63%(P<0.05)。Smad7蛋白在癌旁正常组织中的阳性表达率为19.44%,在口腔鳞癌组织中的阳性表达率为83.33%(P<0.05),且随着分化程度的降低其阳性表达率升高;有淋巴结转移者阳性表达率为92.50%,无淋巴结转移者阳性表达率为68.75%(P<0.05)。结论:Smad4在癌组织中表达明显减少,且分化程度越低,表达越少,Smad4在有淋巴结转移的组织中比在无淋巴结转移的组织中表达低;Smad7的作用则相反。Smad4的表达缺失可能促进了口腔鳞癌的发展和转移,Smad7的过表达促进了口腔鳞癌的发展和转移;BMP/Smads信号传导通路受到抑制,可能会导致口腔鳞癌的继续发生和发展。PURPOSE： To observe the changes of the expressions of Smad4 and Smad7 in oral squamous cell carcinoma and adjacent normal tissue, and to investigate the effects of Smad4 and Smad7 on occurrence and development of oral squamous cell carcinoma （OSCC）. METHODS： Seventy-two cases with OSCC which were pathologically confirmed were included in the study. Cancer tissue and adjacent normal tissue were taken to make slices. SP immunohistochemical method was used to detect the expression of Smad4 and Smad7 in oral squamous cell carcinoma and adjacent normal tissue. SPSSll.5 software package was used for statistical analysis. RESULTS： The positive expression rate of Smad4 protein was 69.44% in adjacent normal tissues, and it was 45.83% in oral squamous cell carcinoma （P〈0.05）. The positive expression rate of Smad4 was lower when the degree of differentiation decreased; The positive expression rate of Smad4 with lymph node metastasis was 22.50%, while it was 65.63% without lymph node metastasis （P〈0.05）. The positive expression rate of Smad7 protein in adjacent normal tissues was 19.44%, and it was 83.33% （P〈0.05） in oral squamous cell carcinoma, the positive expression rate of Smad7 was higher when the degree of differentiation decreased. The positive expression rate of Smad7 with lymph node metastasis was 92.50%, while it was 68.75%（P〈0.05） without lymph node metastasis. CONCLUSIONS： The expression of Smad4 is significantly reduced in eancer tissues and the expression is lower when the degree of differentiation decreased; the expression rate of Smad4 with lymph node metastasis is lower compared with those without lymph node metastasis. Smad7 has the opposite effect. Loss of expression of Smad4 may contrihute to the development and metastasis of oral squamous cell carc：inoma;Over-expression of Smad7 may promote the development and metastasis of oral squamous cell careinoma. The inhibition of BMP/Smads signaling pathway may result in the occurrence and development of oral squamous cell c

关 键 词：骨形成蛋白 口腔鳞癌 SMAD4 SMAD7 信号通路 

分 类 号：R739.8[医药卫生—肿瘤]