Heritable gene-targeting with gRNA/Cas9 in rats  

作　　者：Xinli Hu Nannan Chang Xuelian Wang Fengyun Zhou Xiaohai Zhou Xiaojun Zhu Jing-Wei Xiong 

机构地区：[1]Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Instituteof Molecular Medicine, Peking University, Beijing, China

出　　处：《Cell Research》2013年第11期1322-1325,共4页细胞研究（英文版）

摘　　要：The rat is a preferred animal model in many research applications, especially in physiological, behavioral and translational studies [1]. Although the rat embryonic stem （ES） cells were successfully isolated [2, 3], the rat ES cell-based gene-targeting approach has not been widely adopted due to the technical difficulties in ma- nipulating these cells. The first knockout rat was gener- ated via microinjection of zinc finger nuclease （ZFN） into the embryos [4]. In addition to ZFN, new genome- editing tools such as transcription activator-like effector nuclease （TALEN） [5] and the clustered, regularly inter- spaced, short palindromic repeats （CRISPR）/CRISPR- associated protein （Cas） system [6] have offered a rapid and efficient means of genome modification in many spe- cies. Technologies of ZFN and TALEN have made gene- targeting in the rat genome more convenient and practi- cal [7-9].

关 键 词：基因组 大鼠 ES细胞系 遗传 动物模型 显微注射 编辑工具 转录激活 

分 类 号：Q78[生物学—分子生物学] Q959.837