supported by the National Natural Science Foundation of China (31325026, 31461163006);Natural Science Foundation of Hubei (2015CFA007);the Chinese Academy of Sciences (XDA08010106)
Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination(HR)a...
supported by the National Basic Research Program of China(2012CBA01300 and2012CB966500)
Mouse embryonic stem cells(mESCs)derived from inner cell mass(ICM)of pre-implantation embryos,can maintain undifferentiated state when cultured in N2B27 medium supplemented with GSK3inhibitor CHIR99021 and MEK inhibit...
supported by the National Key Basic Research Program of China(2012CB966500,2013CB-967100);the National Natural Science Foundation of China(31201112)
The androgenetic embyronic stem (aES) cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly...
The rat is a preferred animal model in many research applications, especially in physiological, behavioral and translational studies [1]. Although the rat embryonic stem (ES) cells were successfully isolated [2, 3],...