Derivation of androgenetic embryonic stem cells from m-carboxycinnamic acid bishydroxamide (CBHA) treated androgenetic embryos  

作　　者：SHUAI Ling FENG ChunJing ZHANG HaiJiang GU Qi JIA YunDan WANG Liu ZHAO Xiao-Yang LIU ZhongHua ZHOU Qi 

机构地区：[1]College of Life Sciences, Northeast Agricultural University of China [2]State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences [3]University of Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2013年第23期2862-2868,共7页

基　　金：supported by the National Key Basic Research Program of China(2012CB966500,2013CB-967100);the National Natural Science Foundation of China(31201112)

摘　　要：The androgenetic embyronic stem (aES) cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly different between uniparental derived aES cells and zygote-derived embryonic stem (ES) cells, therefore, the imprinting related cell pluripotency needs further exploitation. Several approaches have been applied in generation of androgenetic embryos and derivation of aES cell lines. Here, we describe a method to generate androgenetic embryos by injecting two mature sperms into one enucleated oocyte. Then these androgenetic embryos were treated with a histone deacetylase inhibitor: m-carboxycinnamic acid bishydroxamide (CBHA). Further, aES cell lines were successfully derived from these treated androgenetic embryos at blastocyst stage. The CBHA could improve not only the quality of androgenetic embryos, but also the efficiencies of aES (CaES) cells derivation and chimeric mice generation. The imprinted gene expression pattern in the CBHA treated embryo-derived aES (CaES) cells was also highly similar to that of zygote-derived ES cells.The androgenetic embyronic stem （aES） cells are useful models in studying the effects of imprinted genes on pluripotency maintaining and embryo development. The expression patterns of imprinted genes are significantly different between uniparental derived aES cells and zygote-derived embryonic stem （ES） cells, therefore, the imprinting related cell pluripotency needs further exploitation. Several approaches have been applied in generation of androgenetic embryos and derivation of aES cell lines. Here, we describe a method to generate androgenetic embryos by injecting two mature sperms into one enucleated oocyte. Then these androgenetic embryos were treated with a histone deacetylase inhibitor： m-carboxycinnamic acid bishydroxamide （CBHA）. Further, aES cell lines were successfully derived from these treated androgenetic embryos at blastocyst stage. The CBHA could improve not only the quality of androgenetic embryos, but also the efficiencies of aES （CaES） cells derivation and chimeric mice generation. The imprinted gene expression pattern in the CBHA treated embryo-derived aES （CaES） cells was also highly similar to that of zygote-derived ES cells.

关 键 词：胚胎干细胞 雄性 ES细胞系 CAES 治疗 CB 印记基因 表达模式 

分 类 号：Q813[生物学—生物工程] Q492