High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases  

作　　者：Ke Feng Hongrui Luo Yongming Li Ji Chen Yaping Wang Yonghua Sun Zuoyan Zhu Wei Hu 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]University of Chinese Academy of Science,Beijing 100049,China

出　　处：《Science Bulletin》2017年第3期162-164,共3页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31325026, 31461163006);Natural Science Foundation of Hubei (2015CFA007);the Chinese Academy of Sciences (XDA08010106)

摘　　要：Genome editing plays an important role in the research of gene functions,gene correction and cell replacement therapy.Traditionally,genome editing is mainly dependent on the technology of homologous recombination(HR)and embryonic stem(ES)cell lines in mammals[1].However,genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms.Recently,the development of zinc finger nucleases(ZFNs),Genome editing plays an important role in the research of gene functions, gene correction and cell replacement therapy. Tradition- ally, genome editing is mainly dependent on the technology of homologous recombination （HR） and embryonic stem （ES） cell lines in mammals [1]. However, genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms. Recently, the development of zinc finger nucleases （ZFNs）,

关 键 词：基因打靶 核酸酶 转录激活 胚胎干细胞 ES细胞系 子样 黄鳝 基因组 

分 类 号：Q953[生物学—动物学]