半胱氨酸蛋白酶抑制剂C在原发性肝癌组织中表达的初步研究  

Preliminary Study on the Expression of Cystatin C in Primary Liver Cancer

作　　者：何訸[1] 吕瑞雪[1] 胥劲[1] 梁利波[2] 张明峰[2] 安振梅[3] 黄亨建[1] 

机构地区：[1]四川大学华西医院实验医学科,成都610041 [2]四川大学华西医院全科医学科,成都610041 [3]四川大学华西医院内分泌代谢科,成都610041

出　　处：《四川大学学报（医学版）》2013年第6期920-923,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81100303,81201343)资助

摘　　要：目的以原发性肝癌患者(未发生临床远处转移)为研究对象,对半胱氨酸蛋白酶抑制剂C(cystatin C)在原发性肝癌组织中的表达做初步研究。方法建立原发性肝癌组(n=41)、肝硬化组(n=24)和正常健康对照组(n=40)。比较肝癌组(癌组织、癌旁组织、远癌组织)与肝硬化组肝组织中cystatin C的表达,比较各组外周血中cystatin C的水平,并同时进一步就癌组织cystatin C表达强度与肿瘤大小、肝内转移情况,及血清甲胎蛋白(AFP)水平做相关性分析。结果原发性肝癌所有癌组织切片中cystatin C表达均呈阳性反应。部分癌旁组织、远癌组织和肝硬化组织切片中有不同程度cystatin C表达。秩和检验结果显示,肝组织中各组cystatin C表达差异具有统计学意义(P<0.01),cystatin C在肝组织中阳性细胞比例、染色强度和以上两者乘积的表达均为:肝癌组织>癌旁组织>肝硬化组织>远癌组织。血清cystatin C水平比较显示,原发性肝癌组与肝硬化组均高于正常健康对照组,差异具有统计学意义(P<0.001),而肝癌组与肝硬化组之间差异无统计学意义(P=0.769)。相关性分析显示,肝癌组织中cystatin C表达程度(阳性细胞比例分值,染色强度分值)同血清AFP水平、肿瘤大小、肝内转移情况无特殊相关。结论在未发生远处转移的原发性肝癌患者,癌组织中cystatin C表达增强,高于癌旁组织、肝硬化组织及远癌组织,同时血清中cystatin C水平也高于正常健康对照。因此检测组织或血中cystatin C表达水平对判断原发性肝癌不同的疾病阶段具有一定意义。Objective To preliminarily investigate the expression of cysteine proteinase inhibitors （5 （cystatm C） in primary hepatic carcinoma. Methods Hepatic tissue samples and peripheral blood samples were collected from 41 cases of primary hepatic carcinoma, 24 cases of cirrhosis and 40 cases of normal control. To primary hepatic carcinoma, three kinds of hepatic tissue samples were harvested, including carcinoma tissue, adjacent non-tumor tissue, and distant normal tissue. The expression levels of cystatin C in hepatic samples and blood samples were measured by immunohistochemistry method and latex enhanced immune turbidimetric method respectively, and the differences of cystatin C expressions were compared in primary hepatic cancer, cirrhosis, and normal control. Furthermore, the relationships of eystatin C expression with tumor size, intrahepatic metastasis, serum AFP level were studied with correlation analysis. Results The expression of cystatin C was positive, in primary hepatic carcinoma. Part of adjacent non tumor tissue, and distant normal tissue and cirrhosis tissues had some degree of cyctatin C expression. Wilcoxon test showed that the differences of cyctatin C expression in different hepatic tissues were statistics significance （P〈0.01）. The proportion of positive cell, staining intensity, and the product of these two was.. carcinoma tissues：〉 adjacent non-tumor tissues：〉 cirrhosis tissues：〉 distant normal tissue. Compared with normal control, primary hepatic carcinoma and cirrhosis both had higher serum cystatin C level （P〈0. 001）, but there was no difference between hepatic carcinoma and cirrhosis （P=0. 769）. The correlation analysis showed that the level of serum cystatin C was not related to serum AFP, tumor size, or intrahepatic metastasis. Conclusion The expression of cystatin C in primary hepatic carcinoma is higher than that of adjacent non tumor tissue, distant normal tissue, and cirrohsis tissue. The serum cystatin C level of primary hepatic carcinoma is higher th

关 键 词：原发性肝癌 肝硬化 半胱氨酸蛋白酶抑制剂C 

分 类 号：R735.7[医药卫生—肿瘤] R965[医药卫生—临床医学]