2种方法提取秋葵多糖工艺研究  

Comparison of two method for extraction technology of okra polysaccharide

作　　者：高愿军[1] 幸美佳 周靖琦 孙学思 侯保清 秦令祥 

机构地区：[1]郑州轻工业学院食品与生物工程学院,郑州450001 [2]漯河食品职业学院,漯河462000 [3]河南帮太食品有限公司,鹤壁458000

出　　处：《食品科技》2013年第11期189-192,共4页Food Science and Technology

基　　金：河南省教育厅科技研究重点项目(13B550160)

摘　　要：利用微波辅助提取和纤维素酶浸提进行秋葵多糖的提取。使用正交分析法对其数据进行分析。结果表明,纤维素酶浸提秋葵多糖较微波浸提具有优势,纤维素酶浸提时各项影响程度:加酶量>酶解温度>酶解pH值>料液比,其最佳工艺条件为:酶解温度45℃、酶解pH4.8、加酶量2%、料液比1:35(g/mL)。在该条件下正交预测的多糖含量为6.32%。比微波辅助多糖提取率在最优工艺平行比较中提高了3.68%。The microwave extraction and cellulase extraction of okra polysaccharide were analyzed by orthogonal analysis. The results showed that optimum condition of okra polysaccharide extraction were as following： the influence degree： cellulose amount〉temperature〉pH, the extraction temperature is 45 ~, pH is 4.8, cellulose amount is 2%, solid-liquid ratio is 1：35 （g/mL）, under these conditions the orthogonal prediction of the polysaccharides was 6.32%, and comparing to microwave extraction, yield of okra polysaccharide was increased by 3.68%.

关 键 词：秋葵 多糖 微波辅助提取 纤维素酶浸提 正交设计 

分 类 号：R284.2[医药卫生—中药学]