正交试验优选大理野生橄榄总多酚的提取工艺  

Optimizing technique of extracting polyphenol from Dali wild olive by orthogonal test

作　　者：罗永会[1] 张翠香[1] 徐春萍[1] 左绍远[1] 刘云春[1] 

机构地区：[1]大理学院基础医学院生物化学与分子生物学实验室,大连671000

出　　处：《食品科技》2013年第11期226-229,共4页Food Science and Technology

摘　　要：目的:优化野生橄榄提取工艺。方法:利用正交试验优选野生橄榄总多酚的最佳提取工艺,以乙醇浓度、提取时间、提取温度、料液比以及提取次数对总多酚含量的影响。结果:野生橄榄总多酚最优提取工艺为:用60%乙醇、以1:20料液、70℃、提取2 h、2次。结论:优选工艺提取的总多酚含量较高,达5.75%,说明该工艺合理可行,为野生橄榄总多酚进一步研究与应用提供理论参考。Objectives： Optimization of extraction technology of wild olive. Methods： The optimum extraction process by orthogonal optimization of wild olive polyphenol, ethanol concentration, extraction time, extraction temperature, the ratio of solid to liquid and extracting times on total polyphenol content. Results： The wild olive polyphenol optimum extraction process. With 60% ethanol, in 1：20 solution, 70 %, extraction of 2 h, 2 times. Conclusion： Higher total polyphenol content of optimum extraction technology, up to 5.75%. It is shown that this process is reasonable and feasible, provides the theory reference and application for further study of wild olive polyphenol.

关 键 词：野生橄榄 总多酚 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]