核桃隔膜不同提取物中黄酮类物质的含量测定  

Content determination of flavonoids in the extracts of dissepiment of Juglans regia

作　　者：王国军[1] 关丽[2] 王幻[1] 冯荣[1] 刘怡娟[1] 唐辉[1] 

机构地区：[1]新疆特种植物药资源教育部重点实验室,石河子大学药学院,石河子832000 [2]新疆生产建设兵团医院药剂科,乌鲁木齐830002

出　　处：《食品科技》2013年第11期289-293,共5页Food Science and Technology

基　　金：兵团科技项目(2011BA055)

摘　　要：目的：建立核桃隔膜黄酮类物质含量的测定方法，并比较不同产地核桃隔膜中黄酮类物质含量的差异。方法：以木犀草苷为对照品，采用分光光度法对核桃隔膜提取物中黄酮类物质的含量进行测定，并比较了60％乙醇回流、索式、超声3种提取方法所得提取物中黄酮类物质的含量。结果：木犀草苷线性范围15．86-47．5711g／mL，回归方程为Y=0．0132X＋0．0156（r=0．9995），平均回收率（n=6）99．43％（RSD=3．02％），60％乙醇超声法所得提取物中黄酮类物质含量最高（42．19％），新疆高于云南核桃隔膜中黄酮类物质的含量。结论：分光光度法可以作为核桃隔膜的质量控制方法之一，用60％乙醇超声法提取所得提取物中黄酮类物质的含量较高。Objective： To develop a method for the determination of total contents of flavonoids in dissepiment of Juglans regia extracts, and compare their contents in dissepiment of Juglans regia from different origins. Methods： The total contents of flavonoids was determined by ultraviolet spectrophotometry by using the standard of luteoloside. The detection was carried out under wavelength of 500 nm, and NaNO2-AI（NO3）3-NaOH as chromogenic agent. Results： The linear ranges of luteoloside was 15.86-47.57 IJg/mL and regression equation was Y=0.0132X＋0.0156（r=0.9995）. The average recoveries of total flavonoids was 99.43% （RSD=3.02%）. The total contents of flavonoids in powder obtained by ultrasonic（42.19%） was higher then that of others, and from Xinjiang above that from Yunnan. Conclusions： Spectrophotometric method can be used for the quality control of walnut diaphragm, and the total contents of flavonoids in powder obtained by ultrasonic（42.19%） is high.

关 键 词：核桃隔膜 黄酮类物质 木犀草苷 分光光度法 

分 类 号：R917[医药卫生—药物分析学]