金黄色葡萄球菌选择培养基成分筛选及优化  

Screening and optimization of Staphylococcus aureus medium

作　　者：薛振菲[1] 李莹[1] 陈晓珑[1] 张晓峰[1] 李文杰[1] 

机构地区：[1]郑州大学公共卫生学院,郑州450001

出　　处：《食品科技》2013年第11期299-303,共5页Food Science and Technology

基　　金：河南省重大公益科研项目(101100911300)

摘　　要：目的:筛选和优化金黄色葡萄球菌选择性培养基成分,为更优培养基的制备提供基础。方法:以适合金黄色葡萄球菌的普通营养琼脂培养基为基础,以平板计数的方法对培养基成分进行筛选,并采用正交设计的方法确定培养基成分的适宜浓度水平。结果:研制的选择性培养基的主要成分:蛋白胨10 g、琼脂15 g、酵母粉5.0 g、氯化钠15 g、亚碲酸钾20 mg、丙酮酸钠5.0g、氯化锂5 g、去离子水1000 mL,另外pH7.4,培养温度37℃。其中亚碲酸钾对试验效果影响最大。结论:研制的金黄色葡萄球菌选择性培养基与Baird-Parker琼脂平板相比,出菌率无显著性差异,为进一步食品微生物快速检测工作奠定了基础。Objective： Screen and optimize the component of Staphylococcus aureus medium, to improve the detection methods of Staphylococcus aureus, Method： Based on the agar, an optimal experimental condition is picked out by a series of optimized experiments using plate count method, and by a group of orthogonal design method. Result： The main component of Staphylococcus aureus medium is： peptone 10 g, agar 15 g, yeast extract 5 g, sodium pyruvate 5 g, sodium chloride 15 g, lithium chloride 5 g, potassium tellurite 20 mg, deionized water 1000 mL. Besides, pH 7.4, 37 ℃. The main factor affecting the performance of medium is potassium tellurite among the conditions. Conclusion： There is no significant difference（P〉0.05） between developed Staphylococcus aureus medium and Baird-Parker agar plate in the plating efficiency. It can be used for the development of rapid Staphylococcus aureus detection methods.

关 键 词：金黄色葡萄球菌 正交试验 培养基优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]