BDE-47对斑马鱼胚胎-幼鱼的急性毒性及氧化应激作用  

Acute Toxicity and Oxidative Stress Effects of BDE-47 on Zebrafish Embryos and Larvae

作　　者：吉贵祥[1] 石利利[1] 刘济宁[1] 吴晟旻[1] 

机构地区：[1]环境保护部南京环境科学研究所国家环境保护农药环境评价与污染控制重点实验室,南京210042

出　　处：《生态毒理学报》2013年第5期731-736,共6页Asian Journal of Ecotoxicology

基　　金：国家高技术研究发展计划(2013AA06A308)

摘　　要：为研究2,2’4＇4-四溴联苯醚（BDE-47）对斑马鱼胚胎-幼鱼急性毒性、氧化应激及细胞凋亡的影响，以受精后3h的斑马鱼胚胎为染毒对象，用概率单位法计算BDE-47对斑马鱼胚胎-幼鱼的96h·LC50；再参照96h-LC50按-定比例级差设置O．25、0．5、1.0、2．0mg·L^-1 4个浓度组和1个对照组（0mg·L^-1）进行96h半静水式毒性试验，检测斑马鱼超氧化物歧化酶（SOD）及过氧化氢酶（CAT）活性、丙二醛（MDA）含量和细胞凋亡情况。结果表明，BDE-47对斑马鱼96h的急性毒性LC50为3．77mmg·L^-1（95％可信区间1．93～1027mg·L^-1）；2．0mg·L^-1剂量组与对照组相比，SOD活性和MDA含量显著增加，且CAT活性与BDE-47染毒浓度之间存在明显的剂量．效应关系；O．5mg·L^-1BDE-47染毒96h后，即观察到斑马鱼幼鱼出现明显细胞凋亡，主要集中于神经管和脑部。研究表明，BDE-47可以影响斑马鱼体内抗氧化防御系统，并能诱导细胞凋亡；BDE-47导致神经组织的氧化损伤可能在动物神经毒性中起重要作用。The purpose of this study is to explore the acute toxicity and oxidative stress of BDE-47 to ze- brafish embryos and larvae. The 96 h acute toxicity test was conducted using 3 hours post-fertilization ze- brafish, and the 96 h-LC50 of BDE-47 for adolescent zebrafish was calculated using Karber＇s method. The 4 days post-fertilization zebrafish was exposed to 025, 0.5, 1.0 and 2.0 mg·L^-1of BDE-47 for 96 hours in semi- static test system to investigate the effect of BDE-47 on SOD and CAT activities, and MDA contents. Acri- dine orange （AO） staining was performed to observe the apoptosis caused by BDE-47. The results showed that the 96 h-LCso of BDE-47 for zebrafish was 3.77 mg·L^-1, with the 95% confidence interval of 1.93-102.7mg·L^-1. The SOD activity and MDA content of zebrafish in 2.0 mg·L^-1 group were significantly higher comparedwith the control group. The CAT activity showed obvious dose-response relationship with BDE-47 concentra- tion. After exposure to 0.5 mg·L^-1 of BDE-47 for 96 hours, significant apoptosis was observed in the larvae, which mainly accumulated in the neural tube and brain. It is indicated that BDE-47 exposure could induce oxi- dative stress and further cause cell apoptosis in larvae. The tissue oxidative damage might be one of the pri- mary mechanisms of neurotoxicity of BDE-47.

关 键 词：2 2’ 4’ 4’-四溴联苯醚 斑马鱼 急性毒性 氧化应激 

分 类 号：X171.5[环境科学与工程—环境科学]