溴化1-十四烷基-3-甲基咪唑对小鼠的肝脏毒性及其作用机制  

The Hepatotoxicity and Mechanism of Bromide 1-Tetradecyl-3-Methyl Imidazole to Mice

作　　者：徐红梅[1,2] 宣怡红[1] 江云[1] 任娜[3] 丁运生[2,3] 吕丹[1] 张毅帆[1] 李小雪[1] 周剑[1] 许凯兵 

机构地区：[1]合肥工业大学医学工程学院制药工程系,合肥230009 [2]合肥工业大学安徽省先进功能材料与设备重点实验室,合肥230009 [3]合肥工业大学化学工程学院,合肥230009

出　　处：《生态毒理学报》2013年第5期791-798,共8页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金(50973025);合肥工业大学大学生创新创业实验(2012CXCY475)

摘　　要：研究离子液体溴化1-十四烷基-3-甲基咪唑盐([C14mim]Br)对小鼠的肝脏毒性及其作用机制。实验设立3个剂量的染毒组(1/16 LD50、1/8 LD50、1/4 LD50)和1个空白对照组,考察昆明种小鼠染毒14 d后,[C14mim]Br对小鼠血清生化指标、肝脏抗氧化酶活性及脂质过氧化产物的影响,并观察肝脏组织的病理变化。与对照组相比,小鼠染毒后,血清中ALT、AST、DBIL和γ-GT明显升高;肝组织受到不同程度的损伤,HSI增大,蛋白质含量降低。当染毒剂量为1/4 LD50时,肝脏中SOD活性和GSHPx活性显著降低,MDA的含量则明显增加。实验结果表明,[C14mim]Br可损伤小鼠的肝脏功能,破坏机体的抗氧化防御系统,造成氧化损伤和脂质过氧化。In order to study the hepatotoxicity of ionic liquid bromide 1-tetradecyl-3-methyl imidazole salt （[C14mim]Br） to mice and its mechanism, three different dosage groups （1/16 LD50, 1/8 LD50 and 1/4 LD50） and one blank control group were set up. After KM mice were treated with [C14mim]Br solution by intragastric ad- ministration for 14 days, the effects of [C14mim]Br on serum biochemical index, histomorphology, antioxidantenzymes and lipid peroxidation in mice liver were observed. Compared with the control group, the levels of al- anine aminotransferase, aspartate aminotransferase, direct bilirubin and ~/-glutamyl transpeptidase in serum of treated mice were all increased obviously. At meanwhile, damaged liver tissue, higher hepatosomatic index and lower protein content were also observed in treated mice. At the 1/4 LDso group, superoxide dismutase activity and glutathione peroxidase content decreased significantly, while the content of malondialdehyde increased obviously. It is indicated that [C14mim]Br could cause damage to liver function of mouse and destroy its an- tioxidant system, leading to oxidative damage and lipid peroxidation.

关 键 词：离子液体 溴化1-十四烷基-3-甲基咪唑盐 肝毒性 小鼠 

分 类 号：X171.5[环境科学与工程—环境科学]