OK-432和IL-2联合用药对C_(57)BL/6小鼠Lewis肺癌的抑制作用及Bcl-2表达的影响  

Effect of combined treatment with OK-432 and interleukine-2 and Bcl-2 expression in Lewis lung cancer in C_57BL/6 mice

作　　者：周洁[1] 司兆学[1] 董红林[1] 梁海清[1] 蔡军伟[1] 单罢安[1] 杨晶[1] 

机构地区：[1]第一军医大学分校病理教研室,广东广州510315

出　　处：《第一军医大学学报》2000年第6期517-518,522,F003,共4页Journal of First Military Medical University

摘　　要：目的 研究A组溶血性链球菌冻干生物制剂OK-432联合白细胞介素2（IL-2）对小鼠C57BL/6Lewis 肺癌（LLC）生长的抑制作用及对Bcl-2表达的影响。方法 将40只C57BL/6荷瘤LLC小鼠分4组，分别用生理盐水、OK-432、IL-2和OK-432联合IL-2处理，观察用药后肿瘤生长的状况, 用抗鼠bcl-2单克隆抗体进行SABC免疫组化技术半定量测定Bcl-2在Lewis肺癌原发灶中的表达。结果 OK-432/IL-2抑制肿瘤作用强，抑瘤率为60%，（P<0.05）。对照组中的Bcl-2的表达明显高于联合用药组（P<0.05）。结论 OK-432与IL-2有明显的协同抗肿瘤活性，其杀瘤过程主要为对瘤细胞的直接或间接攻击，并促进体内多种反应细胞相互作用，其机制可能与抑制Bcl-2基因的表达、诱导瘤细胞的凋亡有关。Objective To study the effect of OK-432 in combination with IL-2 on Lewis lung cancer (LLC) growth and bcl-2 expression in C57BL/6 mice. Methods Forty C57BL/6 mice bearing LLC were divided into 4 groups and injected with 0.9% NaCl solution, OK-432, IL-2 and OK-432/IL-2. The inhibition of tumor growth after the drug administration and the expression of bcl-2 in paraffin-embedded primary tumor tissues were observed with SABC. Results The growth of LLC in the group with combined treatment was significantly inhibited. ( with inhibition rate of 60%, P < 0.05). The expression of bcl-2 in this group was significantly lowered compared with control group (P < 0.05). Conclusions OK-432 and IL-2 has distinct synergetic anti-tumor effect, possibly by attacking the tumor cells directly or indirectly, and the improved interaction of cells due to inhibition of bcl-2 gene expression and induction of tumor cell apoptosis may also contribute to this effect.

关 键 词：肺癌 生物治疗 OK-432 IL-2 BC1-2 

分 类 号：R734.2[医药卫生—肿瘤]