旁观者效应在HSV_1-TK/GCV杀灭肺癌细胞A549中的作用  

Role of bystander effect in the cytocidal activity of HSV_1-TK/GCV against pulmonary carcinoma cell A549

作　　者：何祥梁[1] 蒋小忠[1] 于化鹏[1] 杨振峰[1] 黄颉[1] 陈新[1] 

机构地区：[1]第一军医大学珠江医院呼吸科,广东广州510282

出　　处：《第一军医大学学报》2000年第6期537-538,共2页Journal of First Military Medical University

摘　　要：目的 研究旁观者效应在TK/GCV系统杀灭肿瘤细胞中的作用。方法 转TK基因肺癌细胞A549-TK与亲代细胞A549分别以不同比例、高低密度混合培养，加50 (mol/L GCV作用5 d，MTT法测活。结果 当细胞以高密度接种（104/孔），TK+细胞占20%就能观察到较对照细胞出现明显的生长抑制（P<0.05），而细胞以低密度接种（103/孔），TK+细胞占50%出现较对照细胞明显地生长抑制 （P<0.05）。结论 旁观者效应在TK/GCV系统杀灭瘤细胞中起重要作用，且这一效应与细胞间密切接触有关。Objective To study the role of bystander effect in the cytocidal activity of TK/GCV against tumor cells. Methods Human pulmonary carcinomal cell line and TK gene-transfected A459 cells were cultured together in varied ratio at low and high cell density respectively, treated with 50 mol/L GCV for 5 d. The living cells were counted by MTT assay. Results When the cells were cultured at a high density (104/well) with only 20% TK+cells, significantly higher growth inhibition than that of control cells (P<0.05) was observed. Obvious growth inhibition was observed (P<0.05) in the cell culture at a low density (103/well) only when TK+cell ratio was raised to 50%. Conclusion Bystander effect plays an important role in the cytocidal activity of TK/GCV against tumor cells, and is quite relative to intercellular contact.

关 键 词：肺癌 基因治疗 旁观者效应 MTT测活性 

分 类 号：R734.2[医药卫生—肿瘤] R394[医药卫生—临床医学]