复方枳椇子制剂对小鼠酒精中毒的解救作用  

Detoxication effect of compound preparation of Hovenia dulcis Thunb in mice with alcoholism

作　　者：宋莎莎[1] 路景涛[1] 袁平凡[1] 何伟[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,抗炎免疫药理学安徽省重点实验室,抗炎免疫药物安徽省工程技术研究中心,合肥230032

出　　处：《中国新药杂志》2013年第21期2524-2528,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30973543;81173075);安徽高校省级自然科学产学研项目(KJ2012A178)

摘　　要：目的：考察复方枳楔子制剂（CPH）对急性酒精中毒小鼠神经行为学及乙醇代谢酶活性的影响。方法：昆明种小鼠随机分为正常对照组，模型组，CPH低、中、高剂量组（1，2，4 g·kg^-1），醒酒一号葛根片组（XJGG 4 g·kg^-1），每组10只。给药组灌胃给予相应剂量的药物，正常对照组及模型组给予等量的0.5％羧甲基纤维素钠（CMC-Na），0.5h后，除正常对照组外，其余各组小鼠均灌胃给予56％乙醇（灌胃体积为5 g·kg^-1），在0.5，1，2h时分别检测各组小鼠神经行为学指标；同时使用酶联免疫吸附试验（ELISA）法检测各组小鼠肝组织中乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）、微粒体乙醇氧化酶（EO）活性。结果：与模型组比较，CPH2，4 g·kg^-1剂量组在1，2h自主活动次数减少，在0.5～2h到达参照物的潜伏时间缩短，CPH4g·kg“剂量组在0.5～2h于转棒上的停留时间显著增加；此外，CPH2，4g·kg。能增加饮酒小鼠肝组织中ADH，ALDH，EO活性，其中以CPH4 g·kg^-1剂量组尤为显著。结论：CPH能增加乙醇代谢酶活性，抑制饮酒小鼠中枢神经系统兴奋性，改善乙醇所致的小鼠空间辨别障碍及共济失调现象，具有明显的解酒及中枢保护作用。Objective： To determine the detoxication effects of compound preparation of Hovenia dulcis Thunb （CPH） on the behaviors and activity of ethanol metabolizing enzymes in acute ethanol intoxication in mice. Methods ： KM mice were randomly divided into groups, and treated with CPH （ 1,2, 4 g·kg^-1）, XJGGK （4 g·kg^-1） or equal volume of 0.5% CMC-Na. At 0.5 h after the pretreatment, mice were intragastrically infused with ethanol （56% at 5 g·kg^-1）. Then locomotor activity and the behaviors in Morris water maze and balance beam tests were assessed at 0.5, 1 and 2 h after ethanol ingestion. Meanwhile, the activities of ADH, ALDH and EO in liver tissue were tested by ELISA. Result： Compared with ethanol alone group, CPH 2 and 4 g·kg^-1decreased the locomotor activities at 1 and 2 h, and shorted the latency from 0.5 to 2 h. CPH 4 g·kg^-1 significantly increased the sustaining time in the rotarod from 0.5 to 2 h. Additionally, CPH 2 and 4 g·kg^-1 increased the activities of ADH, ALDH and EO in liver tissue in acute ethanol-intoxicated mice, especially in CPH 4 g·kg^-1 group. Conclusion： CPH can in- crease the activity of ethanol metabolizing enzymes, suppress irritability of the central nervous system, and improve spatial discrimination and ataxia phenomenon of acute ethanol-intoxicated mice. Thus CPH can alleviate alcoholism and has a protective effect on the central nervous system.

关 键 词：复方枳棋子制剂 急性酒精中毒 神经行为学表现 乙醇脱氢酶 乙醛脱氢酶 

分 类 号：R961[医药卫生—药理学]