鼻-鼻窦恶性肿瘤患者T细胞亚群的检测  

Examination of the expression of helper-inducer T-lymphocytes in sino-nasal malignant tumors

作　　者：孙洁[1] 刘勤松[1] 李敬华[2] 袁勇[1] 薛卫国[1] 

机构地区：[1]山东省青岛市市立医院耳鼻咽喉科,266011 [2]青岛市市立医院中心实验室

出　　处：《中国中西医结合耳鼻咽喉科杂志》2013年第5期338-340,共3页Chinese Journal of Otorhinolaryngology in Integrative Medicine

摘　　要：目的检测鼻-鼻窦恶性肿瘤Th1/Th2水平的变化,探讨T淋巴细胞亚群功能紊乱与鼻-鼻窦恶性肿瘤的关系。方法取鼻-鼻窦恶性肿瘤80例,以80例鼻中隔偏曲患者作为对照组,用流式细胞仪测定恶性组与对照组的外周血单个核细胞(PBMC),经佛波酯PMA诱导培养后测定Th1细胞内IFN-γ和Th2细胞内IL-4的水平。结果恶性肿瘤组的CD3+CD8-IL-4+细胞高于对照组(P<0.05),而CD3+CD8-IFN-γ+细胞水平低于对照组(P<0.05),且Th1/Th2比值降低(P<0.05)。结论外周血处于Th2优势分化状态,细胞免疫功能不能有效地激活,从而不利于机体组织以细胞免疫为主的抗肿瘤免疫反应,使鼻-鼻窦恶性肿瘤细胞得以生存发展。Objective To explore the correlation between the unbalanced resevse of Th1/Th2 cytokins and sino-nasal neoplasm by examining the expression percentage of Th1/Th2.Methods 80 patients with malignant tumor in nasal cavities or sinuses were enrolled as experimental group,and 80 patients with deflection of nasal septum were enrolled as control group.The PMA stimulated peripheral blood mononuclear cells（PBMC）were obtained from 80 cases of malignant tumor in nasal cavities or sinuses and 80 normal controls.FCM was used to detect the expression percentage of IL-4 in Th2 cell and IFN-γ in Th1 cell.Results The expression percentage of CD3＋CD8-IL-4＋ cell in malignant tumor group was higher than that in normal controls （P〈0.05）,while the expression percentage of CD3＋CD8-IFN-γ＋ cell in malignant tumor group was lower than that in normal controls （P〈0.05）. Conclusion cellular immuninty function could not be effectively activated as Th2 cells in the advantage state of differentiation,thus antitumor immune response basing on cellular immunity was unfavorable in the body tissue,and then sino-nasal malignant tumor cells could exist and develop.

关 键 词：流式细胞术 鼻腔鼻窦恶性肿瘤 辅助性T淋巴细胞 白细胞介素4(IL-4) 干扰素Ⅱ型(IFN-γ) 

分 类 号：R739.62[医药卫生—肿瘤]