CTP-OD1-HA和CTP-OD2-HA融合肽对白血病K562细胞裸鼠皮下移植瘤的作用  

The effects of CTP-OD1-HA and CTP-OD2-HA fusion peptide on subcutaneous xenograft of leukemia K562 cells in nude mice

作　　者：肖恒[1] 王海霞[1] 陶崑[1] 钟梁[1] 黄世峰[1] 冯文莉[1] 

机构地区：[1]重庆医科大学检验医学院临床血液学教研室,临床检验诊断学教育部重点实验室,重庆400016

出　　处：《肿瘤》2013年第11期954-958,共5页Tumor

基　　金：国家自然科学基金资助项目(编号:30670901;30871102)

摘　　要：目的:探讨细胞质转导肽-寡聚化结构域1-血凝素(cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin,CTP-OD1-HA)和CTP-OD2-HA融合肽对白血病K562细胞裸鼠皮下移植瘤的作用。方法:CTP-OD-HA(阳性对照)、CTP-HA(阴性对照)、PBS(空白对照)、CTP-OD1-HA和CTP-OD2-HA分别预处理K562细胞,并将其分别注射入裸鼠皮下,观察裸鼠皮下移植瘤的生长情况,TUNEL法检测移植瘤组织中肿瘤细胞的凋亡,免疫组织化学法检测肿瘤组织中凋亡相关蛋白Bax和Bcl-2的表达情况。结果:CTPOD1-HA和CTP-OD2-HA处理组移植瘤体积明显小于CTP-HA组(P<0.05),且肿瘤细胞内出现空泡样凋亡改变。TUNEL法检测结果显示,CTP-OD1-HA和CTP-OD2-HA处理组肿瘤细胞的凋亡改变比CTP-HA组更明显(P<0.05)。CTP-OD1-HA和CTP-OD2-HA处理组肿瘤组织中凋亡相关蛋白Bax蛋白的表达水平明显高于CTP-HA组(P<0.05),而Bcl-2蛋白的表达水平明显低于CTP-HA组(P<0.05)。结论:CTP-OD1-HA和CTP-OD2-HA融合肽能够抑制K562细胞在裸鼠体内的致瘤能力,并促进肿瘤细胞凋亡。Objective： To investigate the effects of fusion peptide cytoplasmic transduction peptide-oligomerization domain 1-hemagglutinin （CTP-OD1-HA） and CTP-OD2-HA on subcutaneous xenograft of leukemia K562 cells in nude mice. Methods： K562 cells were pre-treated with CTP-OD-HA （as a positive control）, CTP-HA （as a negative control）, PBS （as a blank control）, CTP-OD1-HA and CTP-OD2-HA, respectively, then the cells were injected into nude mice subcutaneously. The growth of the subcutaneous xenograft was observed. The apoptosis of the tumor cells in subcutaneous xenograft was detected by TUNEL method. The expression levels of Bax and Bcl-2 proteins in subcutaneous xenograft were examined by immunohistochemistry. Results： The volumes of the subcutaneous xenografts in CTP-OD1-HA group and CTP-OD2-HA group were smaller than that in CTP-HA group （P 〈 0.05）, with the change of cavitation apoptosis. The apoptotic change of tumor cells was significant in CTP-OD1-HA group and CTP-OD2-HA group by TUNEL method （P 〈 0.05）. The expression level of apoptosis-associated protein Bax was higher in CTP-OD1-HA group and CTP-OD2-HA group than that in CTP-HA group （P 〈 0.05）, whereas the expression level of Bcl-2 was lower （P 〈 0.05）. Conclusion： CTP-OD1-HA and CTP-OD2-HA fusion peptide can inhibit the tumorigenicity of leukemia K562 cells in nude mice and promote the apoptosis.

关 键 词：白血病 融合蛋白类 bcr—abl 小鼠 裸 细胞质转导肽 

分 类 号：R733.7[医药卫生—肿瘤]