参麦注射液减轻大鼠重症急性胰腺炎相关性肺损伤  

Shenmai injection improves lung injury induced by severe acute pancreatitis in rats

作　　者：于齐宏[1] 杜奕奇[1] 李兆申[1] 谢沛[1] 郭杰芳[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433

出　　处：《第二军医大学学报》2013年第11期1183-1186,共4页Academic Journal of Second Military Medical University

摘　　要：目的观察参麦注射液能否减轻大鼠重症急性胰腺炎(SAP)相关性肺损伤,并初步探讨其相关机制。方法将30只SD大鼠随机分为假手术组、SAP模型组和参麦注射液干预组,每组10只。采用经胰胆管注射5%牛黄胆酸钠诱发SAP大鼠肺损伤模型,参麦注射液干预组在建模前10min经尾静脉注射8mL/kg参麦注射液。模型制作24h后经心脏取血1mL测定血淀粉酶,取胰腺组织及左肺组织用于病理学与免疫组织化学检测;取右肺组织匀浆后检测匀浆液中髓过氧化物酶(MPO)活性及丙二醛(MDA)、肿瘤坏死因子α(TNF-α)与白介素1β(IL-1β)含量。结果假手术组大鼠各项指标未见明显异常。SAP模型组大鼠经胰胆管注射5%牛黄胆酸钠24h后,血浆淀粉酶明显升高,胰腺组织小叶结构严重破坏;肺泡结构破坏,肺泡内大量炎性细胞浸润;肺组织氧化应激指标MPO活性与MDA含量明显升高,炎性指标TNF-α、IL-1β含量亦升高,与假手术组相比差异有统计学意义(P<0.01);肺组织ICAM-1与caspase-3呈强阳性表达。参麦注射液干预组大鼠各项指标均较SAP模型组减轻。结论参麦注射液能减轻SAP相关性肺损伤的原发病及肺损伤,其对肺的保护作用与抗氧化损伤、抗炎症反应及抗凋亡等有关。Objective To investigate the effect of Shenmai injection on severe acute pancreatitis （SAP）-associated lung injury and the possible mechanism in rats. Methods The animals were randomly divided into three groups： sham operation group （sham group, n= 10）, severe acute pancreatitis （SAP group, n= 10） and Shenmai injection treatment group （SM group, n= 10）. The rat model of SAP was induced by injection of 5 %sodium taurocholate to the pancreatic duct. The rats in SM group were given Shenmai injection （8 mL/kg） via tail vein 10 min before sodium taurocholate injecting. 24 h after the model was made, 1 mL blood was drawn for serum amylase detection. Left lung tissues and pancreatic tissues were harvested for pathology and immunohistochemistry. Right lung tissue was homogenated for detection of myeloperoxidase （MPO） activity and levels of malondialdehyde （MDA）, tumor necrosis factor-a （TNF-α） and interleukin-1β （IL-1β）. Results There were no obvious damages to pancreas and lung in the sham group. While in the SAP group, the plasma amylase was greatly increased, the pancreatic lobular structures were severely damaged, and the pulmonary alveoli were destroyed with inflammatory cell infiltration. The MPO activity, MDA, TNF-a, and IL-1β levels in the SAP lung tissues were significantly increased compared with those in the sham group （P〈0. 01）. ICAM-1 and caspase-3 were strongly positive in the lung tissues of SAP group. These parameters in the SM group were improved compared with those in the SAP group. Conclusion Shenmai injection has a protective effect on SAP-associated lung injury, and the mechanism may be related to the inhibition of apoptosis and reduction of oxygen free radical and inflammatory content.

关 键 词：重症急性胰腺炎 肺损伤 参麦注射液 氧化性应激 炎症 细胞凋亡 

分 类 号：R576.1[医药卫生—消化系统]