新疆经典型卡波氏肉瘤患者不同样本HHV-8DNA病毒载量与临床分期关系初步分析  

Relationship between human herpesvirus 8DNA determination of viral loads and disease stage in different samples of classic Kaposi sarcoma patients of Xinjiang

作　　者：王晓东[1] 高翠荣[2] 陈婧弘[1] 王慧[3] 惠艳[1] 

机构地区：[1]新疆医科大学第一附属医院皮肤科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院风湿科,新疆乌鲁木齐830054 [3]新疆医科大学第一附属医院医学研究中心,新疆乌鲁木齐830054

出　　处：《中华肿瘤防治杂志》2013年第21期1638-1642,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：新疆医科大学第一附属医院科研奖励基金(2010YFY19)

摘　　要：目的:了解新疆经典型卡波氏肉瘤(Kapos’s sarcoma,KS)患者皮损、外周血单核细胞(peripheral blood mononuclear cells,PBMC)、唾液和尿液中人疱疹病毒型(HHV8)DNA病毒载量水平及分布情况,探讨HHV8病毒载量与经典型KS临床分期之间的相关性。方法:选取2011-03-09-2012-11-11就诊于新疆医科大学第一附属医院皮肤科8例经典型KS患者的4种类型32份样本中扩增HHV8DNA ORF26基因片段,应用RT-qPCR测定32份样本中HHV8病毒载量,评价载量负荷与经典型KS疾病临床分期之间的关系。结果:全部资料均获完整基因组DNA。新疆经典型KS患者不同样本中HHV8DNA检出率为100%。标准曲线良好,扩增产物特异,其中,HHV8病毒载量负荷皮损组织为205.75±124.02,唾液为70.75±53.01,PBMC为39.88±26.98,尿液为28.25±12.26,差异有统计学意义,F=11.206,P<0.001。HHV8病毒载量水平皮损组织>唾液>PBMC>尿液。结论:新疆经典型KS患者多样本中均有HHV8DNA检出,HHV8病毒载量水平在皮损中最高,在尿液中最低,与新疆经典型KS临床分期之间未呈现相关性趋势。OBJECTIVE： To investigate the presence and distribution of H HV8 DNA viral load in the skin lesions, PBMC,saliva,urine with the classic KS patients of Xinjiang and study the correlation between HHV8 viral load with clas sic KS clinical stage. METHODS： The genomic DNA was extracted from 32 samples of the 4 types from 2011-03 09 to 2012d1-11 in First Affiliated Hospital of Xinjiang Medical University. RT-qPCR was used in determination HHV8 viral load in 32 samples to evaluate the relationship between HHV8 viral load with classic KS clinical stage. RESULTS： All specimens got the complete genomic DNA. HHV8 DNA detection rate was 100% in different samples of the 4 types of classic KS patients. The standard carve parameters had good and specific amplification products. HHV8 viral load showed as follows：lesions〉 saliva〉 PBMC〉 urine. The mean values were 205. 75 ±124.02,70. 75 ±53.01,39.88 ±26. 98, 28.25 ±12.26. Among the four groups, there was statistically significant difference （ F = 11. 206, P 〈 0.001 ）. CONCLU- SIONS： HHV8 DNA viral load is detected in the different samples from classic KS patients in Xinjiang. The HHV8 viral load levels are the highest in the skin lesions, the lowest in the urine, and show no correlation in HHV8 viral load with classic KS clinical stage.

关 键 词：卡波氏肉瘤 人疱疹病毒8型 反转录聚合酶链反应 病毒载量 样本 

分 类 号：R730.2[医药卫生—肿瘤]