Effects of hyperbaric oxygen preconditioning on ischemia-reperfusion inflammation and skin flap survival  

作　　者：Qi Zheng Gao Chun-jin Wang You-bin Ma Xue-mei Zhao Ling Liu Fu-jia Liu Xue-hua Sun Xue-jun Wang Xiao-jun 

机构地区：[1]Beijing Union Med Coll Hosp, Dept Plast Surg, Beijing 100032, Peoples R China [2]Capital Med Univ, Beijing Chaoyang Hosp, Dept Hyperbar Oxygen, Beijing 100020, Peoples R China [3]Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100083, Peoples R China [4]Second Mil Med Univ, Fac Naval Med, Dept Diving Med, Shanghai 200433, Peoples R China

出　　处：《Chinese Medical Journal》2013年第20期3904-3909,共6页中华医学杂志（英文版）

摘　　要：Background Hyperbaric oxygen preconditioning （HBO） is a new method of ischemia preconditioning. In this study, we examined its effects on skin flap survival and the mechanisms involved. Methods Thirty-six rats were divided into three groups： HBO preconditioning, control, and sham groups. An extended epigastric adipocutaneous flap based on the right superficial epigastric artery and vein was raised. A 3-hour period of flap ischemia was induced by clamping the pedicle vessels with a microvascular clamp. At the end of ischemia induction, the clamp was removed and the flap was resutured. Rats in the HBO preconditioning group were treated with HBO four times before surgery. Microcirculation in the skin flap was measured on postoperative days 1, 3 and 5. The size of the flap was measured on postoperative day 5, before the animals were sacrificed. Samples of the skin flap were prepared and stained with hematoxylin and eosin. The levels of tumor necrosis factor （TNF）-α, interleukin （IL）-1β, and IL-6 in the flap samples were measured.Background Hyperbaric oxygen preconditioning （HBO） is a new method of ischemia preconditioning. In this study, we examined its effects on skin flap survival and the mechanisms involved. Methods Thirty-six rats were divided into three groups： HBO preconditioning, control, and sham groups. An extended epigastric adipocutaneous flap based on the right superficial epigastric artery and vein was raised. A 3-hour period of flap ischemia was induced by clamping the pedicle vessels with a microvascular clamp. At the end of ischemia induction, the clamp was removed and the flap was resutured. Rats in the HBO preconditioning group were treated with HBO four times before surgery. Microcirculation in the skin flap was measured on postoperative days 1, 3 and 5. The size of the flap was measured on postoperative day 5, before the animals were sacrificed. Samples of the skin flap were prepared and stained with hematoxylin and eosin. The levels of tumor necrosis factor （TNF）-α, interleukin （IL）-1β, and IL-6 in the flap samples were measured.

关 键 词：skin flap hyperbaric oxygen PRECONDITIONING inflammation factor 

分 类 号：R363[医药卫生—病理学]