RINl在肝癌组织中的表达及对预后和肿瘤侵袭能力的影响  

RIN1 expression in hepatocellular cancer and the affection on prognosis and tumor invasion ability

作　　者：刘海洋[1] 吴刚[1] 何辉[1] 王雅玮[1] 程颖[1] 刘永锋[1] 

机构地区：[1]中国医科大学附属第一医院普通外科教研室肝胆外科,沈阳110001

出　　处：《中华外科杂志》2013年第11期1025-1029,共5页Chinese Journal of Surgery

摘　　要：目的探讨RINl在肝癌中的表达及临床意义。方法应用real—timePCR检测36例配对新鲜肝癌及癌旁组织中RINl基因mRNA表达水平，再用免疫组化方法检测110例肝癌组织石蜡标本RINl蛋白表达水平，观察RINl表达与肝癌患者预后的关系。质粒转染HepG2细胞系，细胞侵袭试验观察RINl对细胞侵袭能力的影响。结果RINl在肝癌组织中表达明显低于配对的癌旁组织（x2=7．430，P=0．026）。免疫组化结果显示RINl蛋白在76例（69．1％）肝癌组织中呈低表达或不表达。生存曲线分析显示RINl低表达患者术后5年生存率（6．46％）明显低于RINl高表达患者（12．96％）（X。=13．808，P〈0．05）。细胞侵袭试验表明RINl过表达能够抑制HepG2细胞侵袭能力（t=8．975、9．522，P〈0．05）。RINl表达与ABL2和E钙蛋白呈正相关（r=0．898、0．912，P〈0．05），与基质金属蛋白酶9呈负相关（r=-0．933，P=0．002）。结论RINl在肝癌组织中呈低表达，与HCC患者预后明显相关，过表达RINl能够抑制肝癌细胞侵袭能力。Objective To test the expression of RIN1 in hepatocellular cancer （HCC） and study its clinicopathological significance and mechanism. Methods RIN1 mRNA in 36 HCC tissues was analyzed using real-time PCR （RT-PCR）. The expression of RIN1 was examined by immunohistochemistry （IHC） in 110 HCC specimens. The relationship between the protein expression and prognosis was analyzed. Transwell was used to test invasion ability of HCC cell lines which were transfected with the expression vector pEGFP- N1-RIN1. Results RIN1 mRNA expression levels was much lower in tumor tissues than that in their corresponding non-cancerous tissues （X2 = 7.430, P = 0. 026）. RIN1 protein was lowly expressed in liver cancer samples （ 69. 1% ） and correlated with poor survival （ 6.46% ） （ X2 = 13. 808, P 〈 0. 05 ）. Transwell assays show that RIN1 overexpression can inhibit invasion ability of HepG2 cells （t = 8. 975 and 9. 522, both P 〈 0. 05 ）. RIN1 expression and ABL2 and E calcium protein were positively correlated （ r = 0. 898 and 0. 912, P 〈 0. 05 ） , and negatively correlated with MMP-9 （r = -0. 933, P = 0. 002）. Conclusions RIN1 expression was down-regulated in HCC and low expression of RIN1 foreshows poor prognosis of HCC patients. RIN1 overexpression can inhibit invasion ability of HepG2 cells.

关 键 词：癌 肝细胞 预后 RIN1 

分 类 号：R735.7[医药卫生—肿瘤]