干细胞转录因子Sox2在肺癌中的表达和意义  

The Expression and Significance of Stem Cell Transcription Factor Sox2 in Lung Carcinoma

作　　者：许伟[1] 位云艳[1] 谭瑶曦 徐玮[2] 程雁[3] 吴剑卿[1] 

机构地区：[1]南京医科大学第一附属医院老年呼吸科,南京210029 [2]江苏省肿瘤医院病理科,南京210009 [3]南京医科大学发育遗传学系,南京210029

出　　处：《中国肺癌杂志》2013年第11期591-595,共5页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金资助项目(No.30971320;No.81272602;No.81302014);江苏省高校自然科学研究项目资助(No.12KJB320004);江苏省干部保健科研课题(No.019);江苏高校优势学科建设工程资助项目资助~~

摘　　要：背景与目的转录因子Sox2维持干细胞的全能性,参与肿瘤干细胞的自我更新,在多种肿瘤的发生发展中发挥重要作用。本研究旨在探讨Sox2及Sox2自身抗体(Sox2-Ab)在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者组织及血清中的表达和意义。方法荧光定量PCR及免疫组化法检测58例NSCLC、16例其他肿瘤和20例正常肺组织标本中Sox2基因及蛋白表达,ELISA法检测30例NSCLC患者和30例健康体检者血清Sox2-Ab水平,结合NSCLC临床病理特点进行数据分析。结果肺癌组织中Sox2 mRNA水平及蛋白阳性表达率均高于其他肿瘤及正常肺组织,差异均有统计学意义(P<0.01),且Sox2 mRNA表达增高同肺癌患者病理类型及肿瘤大小有关,与性别、年龄、肿瘤分化程度和淋巴结转移等无关。血清Sox2-Ab水平在NSCLC患者和正常体检者差异无统计学意义。结论Sox2在NSCLC中有较高的表达,与病理类型、肿瘤大小密切相关,Sox2可能成为肺癌新的标志物及治疗靶点。Background and objective Transcription factor Sox2 remains the pluripotency of stem cell, partici-pates in self-renew of cancer stem cell and plays important role during the initiation and development of various cancers. hTis study intends to investigate the expression and signiifcance of Sox2 and Sox2 autoantibodies （Sox2-Ab） in tissue and serum of patients with non-small cell lung cancer （NSCLC）. Methods Expression of Sox2 gene and protein was tested in 58 cases of NSCLC, 16 patients with other tumors and 20 cases of normal lung tissue specimens by quantitative PCR and immunohis-tochemical assay, respectively. Serum Sox2-Ab level was detected in 30 cases of NSCLC patients and 30 healthy controls by ELISA method. Clinical and pathological data from patients were collected and analyzed retrospectively. Results Expression levels of Sox2 mRNA and protein were higher in patient with NSCLC than other groups, with statistically signiifcant differenc-es （P〈0.01）, respectively. Meanwhile, Sox2 mRNA expression increased in NSCLC patients associated with histological type and tumor size. No signiifcant differences in Sox2-Ab serum levels were found between NSCLC patients and normal subjects. Conclusion Sox2 in NSCLC have a higher expression, which is closely related to histological type and tumor size. Sox2 might use as a potential biomarker and therapeutic target for lung cancer.

关 键 词：非小细胞肺癌 细胞转录因子 MRNA水平 NSCLC 肿瘤干细胞 荧光定量PCR LC患者 ELISA法 

分 类 号：R734.2[医药卫生—肿瘤]