端粒酶反转录酶基因表达与星形胶质细胞活化的相关性  

Correlation of telomerase reverse transcriptional gene expression with astrocyte activation

作　　者：杨明坤[1] 张旭[1] 刘川[1] 吴继生[1] 李德毅[1] 盛伟斌[2] 

机构地区：[1]巴中市中心医院骨科,636000 [2]新疆医科大学第一附属医院脊柱外科

出　　处：《中华创伤杂志》2013年第11期1101-1105,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（81060106）

摘　　要：目的探讨端粒酶反转录酶（telomerase reverse transcriptase，TERT）基因的表达与星形胶质细胞活化的相关性。方法20只雄性SD大鼠（新生3d）培养的星形胶质细胞，按随机数字表法分为4组：A组为活化未转染星形胶质细胞，B组为活化转染星形胶质细胞，C组为未活化星形胶质细胞，D组为活化空白质粒转染星形胶质细胞，每组5只。通过细胞增殖试剂盒（cellcountingkit-8，CCK-8）检测各组细胞的增殖率；免疫细胞化学法检测TERT的表达；RT—PCR法检测各组细胞TERT、胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）基因的表达。结果B组星形胶质细胞增殖能力显著低于A、D、c组（F=43．418，P〈0．01）。TERTmRNA和GFAPmRNA在A、D组中的表达明显高于B、c组，A、D组比较差异无统计学意义；在A、D组中二者呈线性关系（r=0．701，0．704，P〈0．01）；B、C组中二者未见明显相关线性关系（r：0．260，P〉0．05）。TERT蛋白和GFAP蛋白在A、D组中的表达明显高于B、C组，A、D组比较差异无统计学意义。结论TERT基因参与了星形胶质细胞的活化，有促进星形胶质细胞活化的作用。Objective To investigate the relationship between telomerase reverse transcriptase （TERT） gene expression and astrocyte activation. Methods Twenty neonatal 3-day-old male SD rats were used for euhure of the astrocytes. The astrocytes were divided into Group A （ activated, non-trans- letted astroeytes）, Group B （activated, transfected astrocytes）, Group C （unaetivated astroeytes） and Group D （activated, empty plasmid transfected astrocytes） according to the random number table, with 5 rats per group. The cell proliferation rate in each group was detected by cell counting kit-8 （ CCK-8 ） ; TERT expression by immunoeytoehemical method ; expressions of TERT and glial fibrillary acidic protein （GFAP） genes by RT-PCR assay. Results Astrocytic proliferation ability in Group B lowered signifi- cantly as compared with that in Groups A, D and C （F =43. 418, P 〈0.01 ）. Expressions of TERT and GFAP mRNAs in Groups A and D were significantly higher than those in Group B and C, and no signifi- cant difference was found between Groups A and D. Besides, there was a linear correlation between mR- NAs expressions of both genes in Groups A and D （ r = 0. 701, 0. 704, P 〈 0.01 ） , while no significant linear correlation was observed in Groups B and C （ r = 0. 260, P 〉 0.05 ）. Expressions of TERT and GFAP proteins in Groups A and D were markedly higher than those in Groups B and C and no significant difference was found between Groups A and D. Conclusion TERT genes are involved in the activation of astrocytes and exert effect on promoting the activation of astrocytes.

关 键 词：脊髓损伤 小神经胶质细胞 瘢痕 端粒酶反转录酶 

分 类 号：R363[医药卫生—病理学]