349例乳腺癌HER-2基因扩增与蛋白表达相关性研究  

Correlation between HER-2 gene amplification and Her-2/neu protein expression in 349 patients with invasive breast cancer

作　　者：张元[1] 吴宁[2] 何小燕[2] 阮思蓓[1] 罗霞[1] 唐明希[1] 

机构地区：[1]泸州医学院病理教研室,四川泸州646000 [2]四川省德阳市人民医院病理科,四川德阳618000

出　　处：《中国医药科学》2013年第20期16-18,共3页China Medicine And Pharmacy

基　　金：泸州医学院博士启动基金(2011204)

摘　　要：目的了解乳腺癌组织中人表皮生长因子受体2(HER-2)基因扩增与Her-2/neu蛋白表达的一致性与相关性。方法采用荧光原位杂交(FISH)法及免疫组化(IHC)法分别检测349例浸润性乳腺癌患者的HER-2基因扩增与蛋白表达情况,分析二者检测结果的一致性与相关性。结果 FISH与IHC符合率为55.3%,结果存在一致性(Kappa=0.189,P=0.000),呈正相关(r=0.434,P=0.000)。结论 FISH与IHC两种检测结果存在一致性,但IHC(+^+++)患者均应以FISH检测作为评价HER-2基因是否扩增的标准方法。Objective To investigate the correlation between human epidermal growth factor receptor 2（HER-2） gene amplification and Her-2/neu protein expression in invasive breast cancer patients. Methods Using fluorescence in situ hybridization （FISH） and immunohistochemistry（IHC） method, the HER-2 gene amplification and Her-2/neu protein expression status were detected, respectively, and its concordance and correlation in a total of 164 samples of invasive breast cancer tissues. Results The overall coincidence rate of FISH and IHC was 55.3% （Kappa=0.189, P-0.000）, with positive correlation （r=0.434, P=0.000） between them. Conclusion There is a concordance between the Her-2/neu protein expression and its gene amplification. HER-2 gene amplification status should be further detected by FISH in patients with HER-2 protein expression （＋ ~ ＋＋＋）.

关 键 词：乳腺癌 人表皮生长因子受体-2 荧光原位杂交 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]