含季铵盐改性硅酸盐的PMMA树脂材料对白色念珠菌抗菌效果的研究  

Antimicrobial effects of PMMA resin containing quaternary ammonium modified silicates to Candida albicans.

作　　者：李平[1] 张智星[2] 周彬[2] 龚士强[2] 毛靖[2] 

机构地区：[1]江汉大学口腔医学系,湖北武汉430016 [2]华中科技大学附属同济医院口腔医学中心,湖北武汉430030

出　　处：《临床口腔医学杂志》2013年第11期651-654,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81170986)

摘　　要：目的:通过溶胶-凝胶法合成季铵盐改性硅酸盐可聚合抗菌单体,将该抗菌单体整合入聚甲基丙烯酸甲酯(PMMA)树脂,并研究抗菌性PMMA树脂对白色念珠菌的抗菌效果。方法:以正硅酸四乙酯与有机硅烷为前体,合成季铵甲基丙烯酰氧基硅酸盐(QAMS);并用固态29Si交叉极化-魔角旋转核磁共振(29Si CP-MAS NMR)和傅里叶变换红外光谱仪(ATR-FTIR)对QAMS以及整合入PMMA的QAMS进行表征。采用直接接触法(DCT)和菌落形成单位(CFU)计数法评估PMMA树脂对白色念珠菌的抗菌性能。结果:合成的QAMS为有机改性硅酸盐,含有甲基丙烯酰氧基可聚合功能基团;DCT和CFU结果表明含QAMS的PMMA树脂对白色念珠菌具有抗菌效果。结论:QAMS可作为可聚合抗菌单体,赋予PMMA树脂持久的抗白色念珠菌性能。Objectine：To synthesize polymerizable antimicrobial monomer through a silane-based sol-gel route and- characterize the anti-Candida albicans activities of polymethyl methacrylate （PMMA） resin incorporated with aforementioned monomer, nethod：Tetraethoxysilane and trialkoxysilanes were used as precursors to synthesize quaternary ammonium methacryloxy silicate （QAMS）. QAMS before and after incorporation into PMMA resin were characterized with 29Si Cross Po- larization-Magic Angle Spinning Nuclear Magnetic Resonance （29Si CP-MAS NMR）and Attenuated TotalReflection-Fourier Transform Infrared Spectroscopy（ATR-FTIR）.Antimicrobial activities againstCandida albicans of PMMA resins were assessed by Direct Contact Test （DCT） and Colony Forming Unit （CFU） assays. Result：QAMS,as an organically modified silicate,was proved to bear polymerizable methacryloxy group. PMMA resin incorporated with QAMS was antimicrobial to Candida albi- cans,as revealed by DCT and CFU assays. Conchlsion：QAMS could be used as antimierobial monomer that can be copoly- merized with PMMA resin, conferring PMMAresin with anti-Candida albicans activities.

关 键 词：抗菌性 生物膜 有机改性硅酸盐 季铵盐 聚甲基丙烯酸甲酯 

分 类 号：R783.1[医药卫生—口腔医学]