利用共焦显微镜对穿透角膜移植术后散光的研究  

Confocal microscopic analysis of astigmatism following penetrating keratoplasty

作　　者：李绍伟[1] Roger W.Beuerman Stephen C.Kaufman Bryan M.Gebhardt Herbert E.Kaufman 谢立信[1] 

机构地区：[1]山东省医学科学院眼科研究所 [2]美国路易斯安那州立大学眼科中心

出　　处：《眼科研究》2000年第5期436-438,共3页Chinese Ophthalmic Research

摘　　要：目的利用共焦显微镜在活体上对造成角膜移植术后散光的因素进行分析。方法6只有色素兔分为两组接受同种异体部分穿透角膜移植术。一组的供受体角膜边缘钻切整齐,缝线均匀,另一组刻意造成角膜切缘不整齐,缝线深浅不一致。在术后2个月内的不同时间,利用共焦显微镜对角膜切口的愈合及缝线对角膜组织的影响进行观察分析。结果角膜切口整齐对合良好的部位疤痕形成小。角膜切口愈合后再拆除缝线,局部角膜组织的形状仍会发生较大改变。结论首次从活体显微水平上直接形象地显示了角膜切口和缝线对角膜移植后散光的影响,提出临床型共焦显微镜对分析穿透角膜移植术后散光变化的研究有着重要参考意义。ObjectiveTo anal yze the astigmatic changes in the cornea s of rabbits following penetrating kerat oplasty(PKP)by using confocal microscopy in vivo.Methods6 Dutch Belted rabbits were performed allograft PKP in two gro ups .In one group ,the grafts and recipients bed margins were created as regular a s possible,and sutures were placed as ev en as possible.In another group,the corn eal margins and sutures were of an irregu lar nature.Following PKP,animals wer e observed at regular intervals by confo cal microscopy.Photographs and digital i mages of the grafts at the graft host mar gin and the interface on the corneal tis sue by sutures were analyzed.Results Only a slight scar formed around the wound in the first group ,while a large scar was s een in the second group. Sutures removal permitted the stressed tissue to return to a normal orientation.ConclusionsThi s is the first time to show the impact o f the wound healing and the placement of sutures to the astigmatism after PKP in a direct way of images.The confocal mic roscopic images have been proven to be very u seful in the analysis of astigmatic chan ges and variation after PKP.

关 键 词：共焦显微镜检验 穿透角膜移植 散光 

分 类 号：R779.65[医药卫生—眼科]