AMD3100对人肝癌HepG2细胞增殖与AFP表达的抑制作用  

AMD3100 inhibited the expression of alpha fetoprotein and the proliferation of human haptoma HepG2 cells

作　　者：龙儒桃[1,2] 郑奕迎[1] 朱明月[2,3] 李伟[2,3] 马兰[1] 谢协驹[1] 

机构地区：[1]海南医学院病理生理学教研室 [2]海南省肿瘤发生和干预重点实验室 [3]海南医学院分子生物学重点实验室,海南海口571199

出　　处：《海南医学院学报》2013年第11期1483-1486,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(30960153);2013年海南省教育厅重点项目(Hjkj2013-27)~~

摘　　要：目的:检测AMD3100对人肝癌HepG2细胞增殖及其与甲胎蛋白(alpha fetoprotein,AFP)表达的影响并探讨其意义。方法:显微镜观察不同浓度AMD3100对人肝癌HepG2细胞生长的影响;MTT法检测不同时间AMD3100对人肝癌细胞HepG2增殖的影响,用Western blotting分析不同浓度AMD3100干预前后AFP、CXCR4的表达变化。结果:AMD3100对人肝癌HepG2细胞的增殖有明显的抑制作用,且随着浓度的增加抑制作用越明显;给AMD3100处理后HepG2细胞的CXCR4和AFP表达明显减弱。结论:AMD3100对HepG2细胞的增殖有抑制作用,这种作用很可能与抑制AFP的表达有关。Objective. To investigate effects of AMD3100 inhibiting the expression of alpha feto- protein and the proliferation of human haptoma HepG2 cells. Methods. The effects of different concentra- tions of AMD3100 on proliferation of human hepatocellular carcinoma cells HepG2 were observed under microscope, and the effects on proliferation of HepG2 in different time points were detected by MTT~ Western blotting method was applied to detect changes of the expression of AFP and CXCR4; Results. It demonstrated that AMD3100 might inhibit proliferation of HepG2 cells, especially at high concentration （〉1.0 μg/mL）. It also has function of down-regulation the expression of CXCR4 and AFP in HepG2 cells. Conclusion= AMD3100 inhibited the proliferation of human hepatoma cells, HepG2, and this inhibi tion might be relation with the down-regulation the expression of AFP in human hepatocellular carcinoma cells.

关 键 词：AMD3100 甲胎蛋白 肝癌细胞 癌细胞增殖 CXCR4 

分 类 号：R735.7[医药卫生—肿瘤]