谷氨酰胺对缺血-再灌注损伤大鼠肠黏膜炎性反应和通透性的影响  

Effect of glutamine on the intestinal mucosa inflammatory reaction and permeability after ischemia-reperfusion in rats

作　　者：舒晓亮[1] 钟静霞[2] 康凯[1] 柳先丽[1] 徐涵[1] 

机构地区：[1]同济大学附属东方医院临床营养科,上海200120 [2]同济大学附属同济医院儿科,上海200065

出　　处：《中华临床营养杂志》2013年第5期292-299,共8页Chinese Journal of Clinical Nutrition

摘　　要：目的 研究谷氨酰胺（Gln）对缺血-再灌注损伤大鼠肠黏膜炎性反应和通透性的影响.方法 将48只SD大鼠肠系膜上动脉夹闭造成缺血后恢复血流,建立肠缺血-再灌注损伤模型,将造模后的SD大鼠按随机数字表分为对照组（n=24）和模型＋Gln组（n=24）,两组大鼠肠内营养供给量为热量125.4 kJ/ （kg·d）,氮量0.2g/ （kg·d）,模型＋Gln组喂饲肠内营养加3％ Gln,对照组大鼠喂饲肠内营养加3％大豆蛋白,造模后实验持续8d.检测造模前、造模后、实验第3天和第8天大鼠肠黏膜和血浆核因子-κB （NF-κB）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、Gln、D-乳酸（D-LAC）和二胺氧化酶（DAO）的变化.观察小肠黏膜形态学变化.结果 造模后对照组和模型＋ Gln组大鼠肠黏膜NF-κB表达明显高于造模前（75.0％比0.0％,P=0.013; 70.8％比0.0％,P=0.019）；肠黏膜IL-6明显高于造模前[（313.27±75.28） pg/g比（227.52 ±58.13） pg/g,P=0.023; （321.75±74.46） pg/g比（227.52±58.13） pg/g,P=0.043];肠黏膜TNF-α[（241.28±65.29） pg/g、（240.35 ±64.86） pg/g]明显高于造模前[（172.45±33.76） pg/g,P=0.036,P=0.011];血浆IL-6[（150.32±18.74） ng/L、（148.21 ±20.19） ng/L]明显高于造模前[（116.37±14.59） ng/L,P=0.032,P=0.025];血浆TNF-α[（127.62±14.24） ng/L、（123.86±13.75） ng/L]明显高于造模前[（85.18±8.84） ng/L,P=0.018,P=0.035]; D-LAC[（0.46±0.03） mmol/L、（0.51 ±0.04） mmol/L]明显高于造模前[（0.27±0.02） mmol/L,P=0.041,P=0.018]; DAO[（2.76±0.57） U/ml、（2.58±0.51） U/ml]明显高于造模前[（1.52±0.24） U/ml,P=0.015,P =0.037]；而血浆Gln[（0.18±0.01） g/L、（0.21±0.01） g/L]明显低于造模前[（0.39±0.03） g/L,P =0.026,P=0.031].实验第3天和实验第8天,对照组大鼠肠黏膜NF-κB[16例（66.7％）、15例（62.5％）]显著高于造模前[0例（0.0％）,P=0.027,P=0.002]；肠黏膜TNF-α[（226.23±55.35） pg/g、�Objective To study the effect of glutamine （Gln） on the intestinal mucosa inflammatory reaction and permeability after intestine ischemia-reperfusion injury in rats.Methods The rat model of intestinal ischemia-reperfusion injury was established by clamping the mesenteric superior artery and then restoring blood flow.Forty-eight model rats were divided into control group （n =24） and model ＋ Gln group （n =24）according to the stochastic indicator method.Both groups were given enteral nutrition with equal energy and nitrogen [energy 125.4 kJ/ （kg · d） and nitrogen 0.2 g/ （kg · d）].The model ＋Gln group was fed with enteral nutrition plus 3％ Gln,while the control group was fed with enteral nutrition plus 3％ soybean protein.The experiment lasted 8 days after modeling.The intestinal mucosa and the plasma levels of nuclear factor-κB （NF-κB）,tumor necrosis factor-α （TNF-α）,interleukin-6 （IL-6）,Gln,D-LACtic acid and diamine oxidase （DAO） were observed in rats before and after modeling and on the 3rb and 8rd day of the experiment.Changes in the morphology of intestinal mucosa were observed by electron microscopy.Results After modeling in control and model ＋ Gln group,the level of NF-κB in intestinal mucosa [18 cases （75.0％） and 17 cases （70.8％）] were significantly higher than those before modeling [0 case （0.0％）,P =0.013,P =0.019],the level of IL-6 in intestinal mucosa [（313.27±75.28） pg/g and （321.75±76.46） pg/g] were significantly higher than those before modeling [（227.52 ±58.13） pg/g,P =0.023,P =0.043],and the level of TNF-α in intestinal mucosa [（241.28 ±65.29） pg/g and （240.35 ±64.86） pg/g] were significantly higher than those before modeling [（172.45 ±33.76） pg/g,P=0.036,P=0.011].The plasma level of IL-6 [（150.32 ± 18.74） ng/L and （148.21 ±20.19） ng/L] were significantly higher than those before modeling [（116.37 ± 14.59） ng/L,P =0.032,P =0.025],the plasma level of TNF-α [（127.62 ± 14.24） ng/Land

关 键 词：谷氨酰胺 缺血-再灌注损伤 肠黏膜 炎性反应 通透性 

分 类 号：R574[医药卫生—消化系统]