慢性利血平抑郁模型大鼠海马单胺类神经递质的变化  

Monoamine neurotransmitter changes in hippocampus at reserpine induced chronic animal model of de- pression

作　　者：王永志[1] 赵静洁[1] 韩玉[1] 杜仪[1] 李宝金[1] 李杨帆[1] 刘路遥[1] 张一帆[1] 张银凤[1] 李丽[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050

出　　处：《中华行为医学与脑科学杂志》2013年第10期877-879,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：北京市自然科学基金项目（7112037）;北京市中医药科技发展资金资助课题（JJ2011-12,JJ2012-32）

摘　　要：目的探索利血平致慢性抑郁大鼠模型的合适剂量，观察不同剂量利血平对大鼠自发活动和体质量的影响，分析模型大鼠海马单胺类神经递质的变化特点。方法60只大鼠随机分为5组，空白组，利血平0．3mg／kg、0．4mg／kg、0．5mg／kg、0．6mg／kg组，每组n=12；动物造模给予腹腔注射利血平注射液1O（／d（按分组中的给药剂量给予），空白组给予同体积蒸馏水，连续用药14d。分别对大鼠open—field得分和体质量进行测量，酶联免疫法检测大鼠海马单胺类神经递质的水平。结果慢性腹腔注射利血平（0．5、0．6mg／kg）可引起大鼠体质量减轻（P〈0．01，P〈0．05），0．4、0．5、0．6mg／kg组出现抑郁样行为；0．3mg／kg组体质量、open-field得分变化不明显。与空白组体质量[（278．75．4-4．65）]和抑郁open．field评分[（60．3．4-19．9）分]比较，0．5mg／kg组14d大鼠体质量[（256．17±4．89）g]与open—field行为评分[（43．45-8．4）分]差异有显著性俨〈0．01）。0．5mg／kg利血平对7d组抑郁大鼠海马5一羟色胺（5-HT）含量有影响[（2．835-1．09）ng,／ml，P〈0．05]，对14d组5-HT含量无明显影响。与空白组比较，0．5mg／kg组14d腹腔注射利血平对单胺类神经递5一羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NA）影响差异无统计学意义（P〉0．05），但可见5-HT与NA含量呈显著负相关（r=-0．449，P〈0．01）。结论慢性腹腔注射0．5mg／kg利血平能引起大鼠抑郁样行为，利血平对海马单胺类神经递质的影响与用药时间相关。Objective To observe the suitable dosage of reserpine for the establishment of rat model of depression and analyze the changes of monoamine neurotransmitter in hippocampus. Methods 60 rats were ran- domly divided into five groups ： the normal group, the 0. 3 mg/kg group, 0.4 mg/kg group, 0. 5 mg/kg group and 0.6 mg/kg group, and 12 rats in each group. The model groups were intraperitoneally administrated by different dosage of reserpine （ according to the dosage of the grouping） for 14 days and rats in the normal group were injec- ted the same volume of solvent. The body weight was recorded at different days and activity was tested by open- field test at 0,7th and 14th day. Monoamine changes in hippocampus were tested by enzyme- linked immunosorbent assay. Results Reserpine could cause body weight loss （0.5,0.6 mg/kg） and depressive behavior （0.4,0.5,0.6 mg/kg）. The group of 0.4 mg/kg showed no difference in weight and open-field test. Compared with the weight body （ （278.75 -＋ 4.65 ） g） and open-field test （60.3 ＋ 19.9） at the model group, the group of 0.5 mg/kg at 14th showed significant differences in weight （ （256.17 ~4.89）g） and behavior（43.4 -＋ 8.4） （P 〈 0.01 ）. Dosage of 0.5 mg/kg had effect to 5-HT in hippocampus at 7th day （ （ 2.83 ＋ 1.09 ） ng/ml, P 〈 0.01 ） but not 14th day. Compare with the normal group at 14th day,the group of 0.5 mg/kg showed no difference at 5- HT,DA and NA,and there was significant negative correlation between 5-HT and NA（ r= -0.449, P〈0.01）. Conclusion Reserpine of 5 mg/kg can result in the depressive behavior of rats. The effect of reserpine to monoamine neurotransmitter in hippocarnpus has relationship with time.

关 键 词：利血平 抑郁模型 单胺类 神经递质 

分 类 号：R749.4[医药卫生—神经病学与精神病学]