RT-PCR法检测非酒精性脂肪肝细胞中P450 2E1基因的表达与普洱茶、铁观音、红茶的关系  

Effect of Fermented Pu-erh Tea, Tieguanyin and Black Tea on the Expression of Hepatocyte Cytochrome P450 2E1 of Non-alcoholic Fatty Liver in Hyperlipidemia Model Rats

作　　者：刘家奇[1] 邵宛芳[1] 秦廷发[1] 黄业伟[2] 赵宝权[1] 侯艳[1] 

机构地区：[1]云南农业大学龙润普洱茶学院,昆明650201 [2]吉林大学生命科学学院,长春130000

出　　处：《中国农学通报》2013年第30期210-214,共5页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设专项资金资助"黑茶加工岗位"(CARS-23);国家科技支撑计划课题"普洱茶功能成分与保健功效的研究"(2007BAD58B04)

摘　　要：探讨普洱茶(熟茶)、铁观音、红茶对高脂饮食诱发的大鼠非酒精性脂肪肝中P450 2E1基因表达的影响。将Wistar大鼠随机分为正常对照组、高脂模型组、药物组以及(低、中、高剂量)普洱茶(熟茶)、铁观音、红茶组[0.5、1.0、2.0 g/(kg·bw)]。试验大鼠灌胃授试茶样水浸提物35天后采血牺牲,分离血清测定抗氧化指标丙二醛(MDA)、谷胱甘肽(GSH-Px)和超氧化物歧化酶(SOD)的活性;采摘肝脏并进行肝脏病理检测;用RT-PCR检测试验大鼠肝脏细胞中P450 2E1的表达量。与高脂模型组相比,(低剂量组)普洱茶(熟茶)降低MDA效果显著(P<0.05);普洱茶(熟茶)、红茶和铁观音组中GSH-Px酶活力、SOD酶活性显著提高;(高剂量)普洱茶(熟茶)、红茶显著降低P450 2E1表达量(P<0.05);通过试验大鼠病理检测发现,普洱茶(熟茶)和(高剂量组)铁观音能明显改善由高脂饮食引起的肝脏脂肪病变。普洱茶(熟茶)、铁观音、红茶对P450 2E1在非酒精性脂肪肝中的基因表达具有不同程度的积极作用,但普洱茶(熟茶)的作用最显著。The aim was to detect the expression of hepatocyte cytochr ome P450 2E1 in rat nonalcoholic steatosis model. Wistar rats were divided into normal group, hyperlipidemia model group, fermented Pu-erh tea group, Tieguanyin group, low, medium and high dose group of the black tea, which are 0.5 g/（kg · bw）, 1.0 g/ （kg · bw）, 2.0 g/（kg · bw） respectively. All rats were sacrificed after 35 days＇ feeding, serum levels of MDA,GSH-Px and SOD transaminase activity and pathological changes of the liver were observed. RT-PCR method was use to test P450 2E1 expression quantity in rat cells. Compared to the hyperlipidemia model group, the serum levels of MDA in low does group of the fermented Pu-erh tea were significantly decreased （P〈0.05）; Fermented Pu-erh Tea had a better effect on improving enzyme activity of GSH-Px and SOD than those of Tieguanyin and black tea; expression of hepatocyte cytochr ome P450 2El in high does group of fermented Pu-erh tea, black tea significantly decreased （P〈0.05）; through the specimens of rats and pathological detection. The high does group of fermented Pu-erh tea and Tieguanyin group could improve hepatic steatosis caused by high-fat diet significantly. Fermented pu-erh tea, Tieguanyin and black tea had different degrees of positive effect to the expression of hepatocyte cytochrome P450 2E1 in rat nonalcoholic steatosis model. And fermented Pu-erh tea had the most obvious effect.

关 键 词：普洱茶(熟茶) P450 2E1 非酒精性脂肪肝 

分 类 号：S571.1[农业科学—茶叶生产加工]