超声醇提黄花蒿残渣总黄酮的工艺优化及抗氧化活性  

Optimization of Ultrasonic-assisted Extraction of Flavonoids from Artemisia Annua L. Residue and Their Antioxidant Activity

作　　者：熊利芝[1,2] 李子唯 欧阳文[2] 张晓蓉[1,2] 李贵[1,2] 李娜[2] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《精细化工》2013年第11期1223-1228,共6页Fine Chemicals

基　　金：国家自然科学基金(51262008;51202087;51364009);湖南省自然科学基金重点项目(12JJ2005);湖南省科技厅工业支撑计划重点项目(2012GK2017);湖南省教育厅重点项目(12A109);湖南省高校创新平台开放基金项目(13K094);返校博士科研资助经费研究项目(jsdxxcfxbskyxm201111);植物资源保护与利用湖南省高校重点实验室开放基金(JSK201108);大学生研究性学习与创新性实验计划(JSU-CX-2012-39);湖南省重点学科建设项目~~

摘　　要：以提取青蒿素后黄花蒿残渣为原料,采用超声醇提总黄酮。通过单因素实验方法和正交实验方法确定了最佳提取工艺条件;考察所得黄酮纯化物对羟基自由基的清除能力,与抗坏血酸、柠檬酸对比测定其对油脂的抗氧化性能。结果表明,黄花蒿残渣中总黄酮超声辅助提取的最佳提取工艺为:按料液比1∶30(即每克固体物料加入30 mL液体物料,下同)加入体积分数50%的乙醇,在55℃下用200 W超声辅助提取40 min,黄花蒿残渣中总黄酮提取率可达到9.10%。该黄酮纯化物对羟基自由基具有清除能力,随质量浓度的增大而升高;黄酮纯化物对油脂有明显的抗氧化性作用,对植物油的抗氧化能力强于抗坏血酸和柠檬酸,对动物油脂的抗氧化能力稍弱于抗坏血酸而略强于柠檬酸。Abstract ： Using ethanol as extraction solvent, flavonoids were extracted under ultrasonic assistance from Artemisia Annua L. residue where artemisinin had been extracted. The optimal ultrasonic-assisted extraction technology was determined by single factor experiment and orthogonal experiment. Meanwhile, the antioxidant activity of flavonoids against oil and fat was evaluated and compared with that of ascorbic acid and citric acid. The results show that the optimal ultrasonic-assisted extraction technology of flavonoids from Artemisia Annua L. residue are as follows： ethanol volume fraction of about 50% , material-to-liquid ratio of 1：30（30 mL liquid per gram material） , ultrasonic power of 200 W, ultrasonic-assisted extracting time of about 40 min and extraction temperature at 55 ℃. The extraction rate of flavonoids reaches 9. 10% under the optimal ultrasonic-assisted extraction technology. The removal efficiency of hydroxyl radical increases along with the increase of the concentration of purified flavonoids. The purified flavonoids exhibit obvious antioxidant activity against oil and fat. It is superior to both ascorbic acid and citric acid in terms of its effect on oil,while it is somewhat superior to citric acid but inferior to ascorbic acid in terms of its effect on fat.

关 键 词：黄花蒿 总黄酮 提取 抗氧化性 油脂 中药现代化技术 

分 类 号：R94[医药卫生—药剂学]