MiRNA Let-7和Lin28在散发性听神经瘤中的表达及意义  

Expression of MiRNA Let-7 and Lin28 in Sporadic Acoustic Neuroma and Its Significance

作　　者：黄孝文[1] 许胜[2] 褚汉启[1] 王少立[1] 崔永华[1] 张平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉头颈外科,武汉430030 [2]湖北省妇幼保健院耳鼻喉科,武汉430070

出　　处：《华中科技大学学报（医学版）》2013年第5期520-524,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究微小RNA Let-7与Lin28在散发性听神经瘤中的表达及二者之间的关系,探讨其对肿瘤进展可能的影响。方法收集18例经过术后病理证实的散发性听神经瘤组织作为实验组,7例正常胃迷走神经为对照组。采用RT-PCR、免疫组织化学染色方法,检测Let-7d和Lin28A/B mRNA的表达,以及Lin28蛋白的表达。结果 RT-PCR检测显示,Let-7dmRNA表达量在听神经瘤组织显著高于对照神经组织(P<0.001),Lin28A/B mRNA表达量在听神经瘤组织显著低于对照组织(PLin 28A=0.004,PLin28B=0.001)。在听神经瘤组织中,Let-7d与Lin28A/B mRNA的表达存在负相关关系(r=-0.671,P=0.002;r=-0.662,P=0.003)。免疫组织化学检测显示在听神经瘤与正常神经组织中均未发现Lin28蛋白阳性染色。结论在听神经瘤的生长及恶性转化抑制机制中,以微小RNA为主导的Let-7d/Lin28双向负反馈调节可能起重要作用。Objective To investigate the expression of miRNA Let- 7 and Lin28 and their association in sporadic acoustic neuroma in order to explore their role in the development of sporadic acoustic neuroma. Methods Real-time polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of Let-7d and Lin28A/B in 18 sporadic acoustic neuroma tissues （experiment group） and 7 normal gastric vagus nerve tissues （control group）. The relationship between the mRNA expression of Let-7d and Lin28A/B in sporadic acoustic neuroma tissues was analyzed. Immunohistochemical staining was used to detect the expression of Lin28 protein in the two groups. Results RT-PCR showed the mRNA expression of Let-7d was significantly higher in acoustic neuroma tissues than in control group （P〈0. 001）, whereas the mRNA expression of Lin28A/B was significantly lower in acoustic neuroma tissues than in control group （PLin28A=0.004,PLin28B=0.001）. The mRNA expression of Let- 7 d was negatively correlated with that of Lin28A （r= -0. 671,P=0. 002） and Lin28B （r= -0. 662,P=0. 003） in acoustic neuroma tissues. Immunohistochemical staining showed absent staining of I.in28 protein in both the sporadic acoustic neuroma and normal nerve tissues. Conclusion The double negative feedback regulation of miRNA Let-7d and I.in28 may play an important role in growth and malignant transformation inhibition of sporadic acoustic neuroma.

关 键 词：听神经瘤 Let-7d Lin28A Lin28B 

分 类 号：R739.61[医药卫生—肿瘤]