TGF-β1诱导HL-60细胞凋亡后Smads蛋白的变化  

Changes of Smads Protein after HL-60 Cell Apoptosis Induced by TGF-β1

作　　者：姚鲲[1] 何晓薇[1] 李旸[1] 吴斌[1] 杨威[1] 廖爱军[1] 刘卓刚[1] 

机构地区：[1]中国医科大学附属盛京医院血液内科,辽宁沈阳110022

出　　处：《医学临床研究》2013年第10期1946-1949,共4页Journal of Clinical Research

摘　　要：【目的】探讨转化生长因子（TGF-β1）诱导HL-60细胞凋亡后Smads蛋白的变化。【方法】用不同浓度的TGF-131处理HL-60细胞，采用MTT法检测细胞生长抑制率，流式细胞仪检测细胞凋亡，RT-PCR方法检测Smads蛋白。【结果ITGF-β1对HL-60细胞有增殖抑制作用，其抑制效应呈时间及剂量依赖性，10．4ng／mL TGF-β1增殖抑制作用较为明显；在细胞凋亡过程中，Smad3、Smad7表达呈逐渐升高趋势（P〈0．05），而Smad2、Smad4则无明显变化。【结论】TGF-β1可抑制HL-60细胞增殖并诱导其凋亡，且呈时间、剂量依赖性；Smad3、Smad7可能参与TGF-β1诱导HL-60细胞凋亡过程，其变化与HL-60细胞凋亡密切相关。[Objective] To explore the changes of Smads protein after HL-60 cell apoptosis induced by TGF-β1. [Methods] Different concentrations of TGF-β1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. Cell apoptosis was detected with flow cytometry and Smads protein was detected with RT-PCR. [Results] TGF-β1 had inhibitory effect on the proliferation of HL-60 cells, and the inhibitory effect was time- and dose-dependent. The effect of 10.4ng/ml TGF-β1 was more obvious. In the course of cells apoptosis, the expression of Smad3 and Smad7 increased gradually（ P 〈0.05）, but Smad2 and Smad4 had no apparent change. [Conclusion] TGF-β1 can inhibit HL-60 cell proliferation, and the inhibition effect is time- and d0se-dependent. Smad3 and Smad7 may be involved in HL-60 cell apoptosis induced by TGF-β1. Their changes are closely related with HL-60 cell apoptosis.

关 键 词：转化生长因子B1 细胞凋亡 HL-60细胞 

分 类 号：R36[医药卫生—病理学]