孕鼠补充牛磺酸对宫内生长受限胎鼠脑组织牛磺酸水平的影响  

Effect of antenatal taurine supplementation on the taurine levels in brain of fetal rat with intrauterine growth restriction

作　　者：滕慧云[1] 李芳[1] 刘敬[2] 王晓凤[2] 刘颖[2] 王华伟[2] 

机构地区：[1]中国人民解放军第二炮兵总医院儿科,北京100088 [2]北京军区总医院附属八一儿童医院新生儿重症监护中心

出　　处：《中华实用儿科临床杂志》2013年第20期1579-1581,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（81170577）

摘　　要：目的探讨宫内生长受限（IUGR）胎鼠脑组织牛磺酸水平及孕鼠补充牛磺酸对IUGR胎鼠脑组织牛磺酸水平的影响。方法采用全程低蛋白饮食法建立IUGR胎鼠模型，将15只孕鼠随机分为3组：正常对照组、IUGR组、IUGR＋孕鼠补充牛磺酸组（牛磺酸组）。牛磺酸组自怀孕第12天起饮食中添加牛磺酸300mg／（kg·d）直至自然分娩。每窝随机抽取2只胎鼠断头取脑，采用液质联用串联质谱法检测各组胎鼠脑组织牛磺酸水平。结果正常对照组、IUGR组与牛磺酸组胎鼠平均出生体质量分别为（6．619±O．413）g、（4．509±0．454）g、（5．176±0．436）g，组间差异有统计学意义（F=429．818，P〈0．01）。正常对照组、IUGR组与牛磺酸组胎鼠脑组织牛磺酸水平分别为（2．399±0．134）×10μg/g、（1．881±0．166）×10μg／g和（2．170±0．191）×10μg／g，组间差异有统计学意义（F=24．828，P〈0．01）。结论孕鼠补充牛磺酸可增加IUGR胎鼠脑组织内牛磺酸水平。Objective To examine the taurine levels and determine the influence of antenatal taurine on the taurine content in the brains of fetal rats with intrauterine growth restriction （IUGR）. Methods Fifteen pregnant rats were randomly divided into 3 groups ： normal control group, IUGR group and the IUGR ± antenatal taurine supplement group（ taurine group） （n = 5）. IUGR models were induced by low protein diet throughout gestation period. Taurine was added to the diet of the taurine group with a dose of 300 mg/（ kg ± d） from 12 days after conception until natural delivery. Two fetal rats were randomly selected from each nest and were sacrificed to obtain the brains, and the tanrine levels in fetal rat brains were detected by high performance liquid chromatography-mass spectrometry. Results The average weight of fetal rats in the normal control group,the IUGR group and the taurine group were （6.619 ±0. 413） g, （4.509 ±0.454） g, （5. 176 ±0. 436） g,there was a significant difference among the 3 groups（F =429. 818,P 〈 0. 01 ）. The taurine levels in fetal rat brain in the normal control group, the IUGR group, and the taurine group were （2. 399 ± 0. 134） x 103 μg/g, （ 1. 881 ± 0.166） x 10s Ixg/g and （2. 170 ± 0. 191 ） x 103 ixg/g, there was a significant difference among the 3 groups （ F = 24. 828, P 〈 0.01 ）. Conclusion Antenatal tanrine supplementation can signifi- cantly increase the taurine level in fetal rat brain with IUGR.

关 键 词：宫内生长受限 牛磺酸 脑 液质联用串联质谱 胎鼠 

分 类 号：R714.5[医药卫生—妇产科学]