南方红豆杉水提物抑制EGFR/MAPK通路诱导人肺癌A549细胞凋亡的研究  

Rearch on aqueous extract of Taxus chinensis var.mairei(AETC) induces apoptosis of human lung carcinoma A549 cells through EGFR/MAPK pathway

作　　者：舒琦瑾[1,2] 谢长生 屠小龙[1,3] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310006 [2]浙江省中医院肿瘤科,杭州310006 [3]宁波市中医院肿瘤科,宁波315010

出　　处：《中华中医药杂志》2013年第11期3412-3416,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然基金项目(No.Y2081051);浙江省中医药科技计划重点项目(No.2009ZA003)~~

摘　　要：目的:进一步研究南方红豆杉水提物抑制人肺癌A549细胞增殖,诱导其凋亡的机制。方法:采用MTT法检测南方红豆杉水提物对A549细胞增殖的抑制作用;流式细胞仪检测其对A549细胞凋亡的影响;Western Blot技术检测其对EGFR/MAPK信号通路相关蛋白表达的影响。结果:南方红豆杉水提物对A549细胞的增殖有抑制作用,且呈剂量效应相关性,IC50值为2.20mg/mL。2.0、2.5、3.0mg/mL南方红豆杉水提物处理24h后凋亡率分别为20.9%,38.0%和61.7%。南方红豆杉水提物抑制磷酸化EGFR和ERK1/2的水平,促进p38 MAPK磷酸化。但对EGFR、ERK1/2和p38 MAPK的表达却无影响。结论:南方红豆杉水提物通过多靶点抑制人肺癌A549细胞增殖,抑制EGFR/MAPK信号通路诱导其凋亡是其机制之一。Objective： The study aimed at further investigating the inhibition efficacy of aqueous extract of Taxus chinensis var.mairei （AETC） on human lung cancer A549 cells proliferation and induce its apoptosis and the possible mechanisms. Methods： MTT method was used to detect the inhibition of AETC on A549 cell proliferation inhibition. Flow cytometry assays were performed to evaluate the effects of AETC on the apoptosis of A549 cells. Western Blot analysis was applied to detect the related protein expression of EGFR/MAPK signal pathway. Results： AETC inhibiter the proliferation of A549 cells in a concentration-dependent manner. The median inhibition concentration （IC50） values were 2.20mg/mL. The apoptotic rates of 2.0,2.5,3.0mg/mL AETC treatments for 24h were 20.9%, 38.0% and 61.7%, respectively. AETC treatment strongly reduced the level of phospho-EGFR and phospho-ERK1/2 while increased phospho-p38. However, no significant effects on the expression of EGFR, ERKI/2 and p38 MAPK were detected. Conclusion： AETC inhibites the proliferation of A549 ceils through multiple targets. Inhibiting EGFR/MAPK signaling pathways induce its apoptosis is one of its possible mechanism.

关 键 词：南方红豆杉 人肺癌A549细胞 凋亡 EGFR MAPK通路 

分 类 号：R285.5[医药卫生—中药学]