番茄红素对UVA氧化损伤皮肤成纤维细胞的保护作用  

Protective Effects on Dermal Fibroblasts Under the UVA Oxidative Stress by Lycopene

作　　者：杨娜[1] 李明[2] 胡家栋[1] 姜涛[2] 巩思嘉 张剑[3] 

机构地区：[1]中国药科大学药学院,南京211198 [2]中国药科大学中药学院,南京211198 [3]中国药科大学中药复方研究室,南京211198

出　　处：《生物技术通报》2013年第11期142-147,共6页Biotechnology Bulletin

基　　金：国家基础科学人才培养基金项目(J0630858);全国大学生创新实验计划

摘　　要：以小鼠皮肤成纤维细胞为材料,给予5 J/cm2强度UVA照射建立模型,通过MTT法检测细胞增殖活力,同时检测细胞内超氧化物歧化酶SOD(Superoxide dismutase)、过氧化氢酶CAT(catalase)、谷胱甘肽过氧化物酶GSH-PX(glutathione peroxidase)和丙二醛MDA(malondialdehyde)的活力,观察细胞内氧自由基含量和清除能力,探索番茄红素对UVA氧化损伤皮肤成纤维细胞保护作用。结果表明,番茄红素保护组较模型组细胞死亡率降低,SOD、CAT、GSH-PX活力上升,脂质过氧化物MDA含量下降,自由基清除能力有明显改善,说明番茄红素可改善成纤维细胞中自由基清除酶活力,提高成纤维细胞对紫外损伤的抗氧化能力。The aim of this study is to test the protective effects of lycopene on the dermal fibroblasts under UNV oxidative stress. The model used in this experiment was established by exposing the mouse dermal fibroblasts to UVA （ 5 J/cm2 ） in X min and the cell proliferative activity was detected by MTF method. The activities of superoxide dismutase （ SOD ） , catalase （ CAT ） , glutathione peroxidase （ GSH-PX ） and malondialdehyde （ MDA ） were also tested to illustrate the protective effect of lycopene. Results showed that the mortality of dermal fibroblasts was improved by subjecting the lycopene to the culture while the cells exposure in the UVA and the activity of SOD, CAT and GSH- PX increased significantly while the the level of lipid hydroperoxide, MDA decreased. In general, lycopene can protect the dermal fibroblasts against the UVA radiation by improving the activity of enzymes which involved in the clearacc of oxygen radicals.

关 键 词：番茄红素 成纤维细胞 UVA 抗氧化 

分 类 号：R285[医药卫生—中药学]