一株高效驱油菌株HB-2降解原油活性的研究  

Activities of an Oil Degrading Bacterial Strain HB-2

作　　者：游靖[1,2] 李青[2] 刘洋[3] 吴刚[2] 王冠[2] 赖丰利[2] 李勇斌[2] 段丽莎[2] 曹艳花[3] 

机构地区：[1]华东理工大学,上海200237 [2]华北油田采油工程研究院,任丘062552 [3]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100027

出　　处：《生物技术通报》2013年第11期170-174,共5页Biotechnology Bulletin

基　　金：中国石油天然气股份有限公司华北油田分公司重大科技项目(2012-HB-z231);中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

摘　　要：以原油为唯一碳源,从华北油田油井产出液中分离筛选得到一株高效降解原油菌株HB-2,经本研究组前期的多相分类鉴定,已将其确定为藤黄色单胞菌(Luteimonas)属中的一个新种华北藤黄色单胞菌(Luteimonas huabeiensis)。该菌与原油作用后,降黏率达47.3%,降低油水界面张力38.7%,能代谢一定量的有机酸和表面活性剂,通过原油组分分析,使原油轻质组分增加,有效地改善了原油的流动性,通过室内物模试验,提高采收率高达15.4%。An oil degrad!ng strain HB-2 was isolated from the produced liquid of oil wells in Huabei Oilfield, which used crude oil as sole carbon. The strain was identified as a new species of the genus Luteimonas by the method of polyphasic identification. Through the reaction with crude oil, we find the viscosity-reducing rate is 47.3% and the oil-water interfacial tension decrease by 38.7%, in addition, the strain can also produce organic acid and surfactant. According to analyzing components of crude oil, we find the strain can improve the fluidity of crude oil by increasing light component of oil. According to the result of indoor model test, we can find the recovery ratio is increased by 15.4%.

关 键 词：油井产出液 华北藤黄色单胞菌(Luteimonas huabeiensis)乳化降黏物模试验 

分 类 号：X172[环境科学与工程—环境科学]