Ezrin蛋白表达与青海汉族和回族胃癌易感性关系研究  

Relationship between the susceptibility and Ezrin protein expression in gastric cancer and normal gastric mucosa of Qinghai Han-and Hui (muslim)-Chinese

作　　者：苏占海[1] 王荣华[2] 杨生玺[3] 侯成荣[3] 陈娟[4] 孟楠[4] 

机构地区：[1]青海大学医学院生物教研室基础医学研究中心,青海西宁810001 [2]青海大学附属医院肿瘤放疗科,青海西宁810001 [3]青海大学医学院生物教研室,青海西宁810001 [4]青海大学医学院临床医学系,青海西宁810001

出　　处：《中国现代医学杂志》2013年第27期47-53,共7页China Journal of Modern Medicine

基　　金：国家自然基金(No:81160259);青海省科技厅项目基金(No:2010-Z-721)

摘　　要：目的研究ezrin蛋白表达与青海汉族、回族胃癌易感性关系;同时研究ezrin对胃癌细胞形态、骨架和增殖的影响。方法利用免疫组化对正常胃黏膜组织和胃癌组织进行ezrin蛋白表达检测,用Western blot蛋白印迹法检测两者ezrin蛋白表达水平,同时利用细胞转染及免疫荧光标记方法检测ezrin对胃癌细胞骨架调节的作用,而细胞增殖则用MTT方法进行检测分析。结果 ezrin蛋白在青海汉族、回族胃癌组织中表达比正常胃黏膜组织中高,具有显著性差异;同时ezrin蛋白表达水平与青海汉族、回族胃癌分化程度、淋巴结转移以及组织学分型有关,而和年龄、性别无关。此外,ezrin蛋白表达水平与青海汉族、回族民族无关,即无民族特异性。蛋白印迹法检测结果显示,肿瘤组织中ezrin蛋白表达水平比正常组织中较高。而细胞免疫荧光检测中,发现ezrin对细胞骨架、细胞形态及细微结构的调节起重要作用,同时MTT检测发现,ezrin转染的胃癌细胞增殖受到一定抑制。结论 ezrin蛋白在青海汉族和回族胃癌组织中表达水平比正常胃癌组织高,同时与胃癌分化程度、组织学分型和淋巴结转移有关;ezrin蛋白表达水平与汉族、回族不同民族无关。由于ezrin转染细胞在细胞形态、骨架和微绒毛等结构方面比对照组变化大,提示Ezrin蛋白与胃癌转染及侵移有关。[Objective] To explore the relationship between the susceptibility and ezrin protein expression in gastric cancer of Qinghai Han- and Hui （muslim）-ethnic group. Meanwhile, we want to investigate the influences of ezrin on the cell morphology, cell polarization and cell proliferation of gastric cancer. [ Methods ] Immunohisto- chemicial staining is used to detect the expression of ezrin protein in normal gastric mucosa and gastric cancer tis- sues. Western blot is used to detect the ezrin expression level in both normal and gastric cancer tissues. Cell trans- fection and immunofluoreseent staining are used to observer the cell morphology change and cell polarization. Cell proliferation is analyzed by MT＇F method. [ Results ] Ezrin protein expression is much higher in the gastric cancer than normal gastric mucosa in both Han- and Hui-ethnic patients of Qinghai Province, and the expression level ofezrin is assoeiated with differentiation grade, lymph node metastasis, and histological type of gastric eancer in both Hart- and Hui （muslim）- ethnics. Through Western blot analysis, it shows ezrin protein expression in normal gastric mucosa is lower than gastric cancer. Furthermore, the protein expression level of ezrin is not related with Han- or Hui （muslim）-ethnic, that means that ezrin expression in gastric cancer is not associated with ethnic speicificity. Ezrin induces cell morphological change, more polarization and microvilli development, and meanwhile it inhibits cell proliferation. [ Conclusions ] The expression of ezrin protein is higher in gastric cancer than normal gastric mu- cosa, and it is also associated with the differentiation grade, lymph node metastasis, and histological type of gastric cancer in both Han- and Hui （muslim）-ethnic groups of Qinghai Province. Ezrin protein expression is not related with the ethnic Han- or Hui （muslim）-ethnic in Qinghai Province. Compared with the control, ezrin stimulates cell morphological change, cytoskeleton remodeling and microvilli development

关 键 词：EZRIN 胃癌 易感性 汉族和回族 细胞骨架 

分 类 号：R735.2[医药卫生—肿瘤]