应用荧光原位杂交技术检测肺癌针吸标本中超二倍体肿瘤细胞  

Detection of Hyperdiploid in Malignant Cells in Fine- needle Aspirates from Lung Cancer by Fluorescence in situ Hybridization

作　　者：贾东辉[1] 张智慧[1] 刘树范[1] 程书钧[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院临床细胞学室,北京100021

出　　处：《中国医学科学院学报》2000年第6期589-591,共3页Acta Academiae Medicinae Sinicae

基　　金：国家重点基础性研究 973项目基金!(G1998051200)

摘　　要：目的探讨肺癌针吸标本中肿瘤细胞染色体数目的异常变化。方法用 7号、 11号、 17号、 X染色体特异的着丝粒 DNA探针，对 30例肺癌针吸标本（ 6例为肺癌原发灶标本， 24例为颈淋巴结标本）进行双色荧光原位杂交。结果 27例肺癌阳性针吸标本中 7号、 X、 17号、 11号染色体超二倍体的病例分别达到 81.5％ (22/27)、 77.8％ (21/27)、 70.4％ (19/27)、 63.0％ (17/27)。 3例肺癌阴性针吸标本中 4个染色体均未出现超二倍体改变。结论 (1)用荧光原位杂交技术对针吸标本进行细胞遗传学分析是一种可行、简单的方法。 (2)肺癌针吸标本中肿瘤细胞染色体超二倍体改变对诊断良、恶性细胞具有一定的参考价值。To study the numerical abnormality of chromosomes in malignant cells in fine- needle aspirates from lung cancer by fluorescence in situ hybridization (FISH). Methods 30 fine- needle aspirates from lung cancer（ 4 from lung tissues, 26 from lymph nodes） were detected by FISH with the centromere DNA probes for chromosome 7,11,17 and X. Results In 27 positive fine- needle aspirates the rates of hyperdiploid in chromosome 7, X, 17， and 11 were 81.5％ (22/27), 77.8％ (21/27), 70.4％ (19/27) and 63.0％ (17/27), respectively. In 3 negative fine- needle aspirates, the number of chromosomes was normal. Conclusions (1) Interphase cytogenetic cells analysis of fine- needle aspirates by FISH is feasible and simple; (2) Hyperdiploid in malignant cells in fine- needle aspirates from lung cancer can be used as a biomarker for benign and malignant cells.

关 键 词：荧光原位杂交 肺癌 超二倍体 

分 类 号：R734.204[医药卫生—肿瘤] R730.43[医药卫生—临床医学]