片叶苔素D大鼠体内代谢产物的结构鉴定  

Structural determination of riccardin D's metabolites in rats

作　　者：凌霄[1,2] 郭东晓[2] 娄红祥[1] 

机构地区：[1]山东大学药学院天然药物化学研究所,济南250012 [2]山东省食品药品检验所,济南250101

出　　处：《山东大学学报（医学版）》2013年第11期56-60,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30730109);国家杰出青年科学基金(30925038)

摘　　要：目的研究片叶苔素D在大鼠体内的代谢,并对片叶苔素D在大鼠体内代谢产物进行分离与结构鉴定。方法大鼠按40 mg/kg剂量口服片叶苔素D后收集胆汁,采用高效液相色谱仪分离、纯化代谢产物,利用HPLCMS/MS分析代谢产物,利用NMR鉴定结构。结果大鼠口服片叶苔素D后,在胆汁中检测到2个Ⅱ相代谢产物,均为片叶苔素D与葡萄糖醛酸的结合产物(片叶苔素D-1’-O-β-D-吡喃葡萄糖醛酸),且两个代谢产物互为旋阻异构体,室温下可互相转化。结论片叶苔素D在大鼠体内产生与葡萄糖醛酸结合的Ⅱ相代谢产物,且2个代谢产物互为旋阻异构体。Objective To investigate the metabolism pathway and identify the metabolites of riccardin D in vivo after oral administration in rats. Methods Using HPLC, the metabolites were purified from rats′ bile samples after oral administration of riccardin D 40mg/kg in rats. The structures of the metabolites were identified by HPLC-MS/MS and confirmed by NMR. Results The riccardin D′s metabolites were the conjugates with glucuronate and were identified as a mixture of two atropisomers. Conclusion The riccardin D′s main metabolism pathway in vivo is phase Ⅱ metabolism and the metabolites are the conjugate with glucuronate.

关 键 词：片叶苔素D 代谢产物 大鼠 

分 类 号：R574[医药卫生—消化系统]