迷走神经刺激对癫痫大鼠海马缝隙连接蛋白43表达的影响  

Effect of vagus nerve stimulation on expression of Connexin 43in hippocampus of rat model of epilepsy

作　　者：冯硕[1] 焦金菊[1] 林宇涵[1] 刘敏杰[1] 郭佳[1] 

机构地区：[1]辽宁医学院生理教研室,辽宁锦州121000

出　　处：《中国生物制品学杂志》2013年第11期1599-1602,共4页Chinese Journal of Biologicals

基　　金：辽宁省教育厅基金资助项目(L2010273)

摘　　要：目的观察迷走神经刺激（vagus nerve stimulation，VNS）对海人酸（kainic acid，KA）致癫痫大鼠海马星形胶质细胞缝隙连接蛋白43（Connexin43，Cx43）表达的影响，探讨Cx43与癫痫之间的关系及其在VNS治疗癫痫中的作用。方法将SD大鼠随机分为对照组、KA组和VNS＋KA组，每组10只；KA组和VNS＋KA组大鼠左侧脑室注射3μl0．05％KA溶液，制备癫痫模型，对照组注射等量生理盐水；VNS＋KA组行VNS。观察各组大鼠行为变化，记录皮层脑电图（electroeortieogram，ECoG）；采用免疫组织化学法和Westernblot法检测各组大鼠海马星形胶质细胞Cx43的表达。结果大鼠左侧脑室注射KA3～5min后，KA组大鼠为RacineⅣ～Ⅴ级重型癫痫发作，脑电图可见棘波、棘慢波及簇状高尖波等异常脑电发放；VNS＋KA组大鼠为RacineⅠ～Ⅲ级轻型发作，脑电发放较KA组减轻，对照组无癫痫发作及异常脑电发放。KA组大鼠海马Cx43阳性细胞数和相对表达量均明显高于对照组和VNS＋KA组（P均〈0．01）。结论癫痫大鼠海马Cx43的表达与癫痫发病机制密切相关，VNS可降低癫痫大鼠海马Cx43的表达，抑制癫痫发作。Objective To observe the effect of vagus nerve stimulation （VNS） on expression of Connexin 43 （Cx43） of astrocytes in hippocampus of rat with epilepsy induced by kainic acid （KA）, and investigate the relationship between Cx43 and epilepsy as well as role of Cx43 in treatment of epilepsy by VNS. Methods SD rats were randomly divided into control, KA and VNS ＋ KA groups, 10 for each. The rats in KA and VNS ＋ KA groups were injected with 3 μl of 0. 05% KA into lateral ventricle to establish the model of epilepsy, while those in control group with an equal volume of physiological saline. Afterwards, the rats in VNS ＋ KA group were subjected to VNS. The rats in various groups were ob-served for behavioral change, recorded for electrocorticogram （ECoG）, and determined for expression of Cx43 in hip-poeampus. Results Epileptic motor seizures and epileptic discharges, such as spike wave, sharp wave and spike （sharp）-slow wave were observed in the rats 3 - 5 rain after injection with KA, with Racine scores of Ⅳ- Ⅴ. However, the Racine scores of rats in VNS ＋ KA group were Ⅰ - Ⅲ, while the discharge was relieved as compared with those in KA group. No epileptic discharge was observed in control group. Both the Cx43-positive cell count and relative expression level of Cx43 in KA group were significantly higher than those in control and VNS ＋ KA groups （each P 〈 0. 01）. Con-clusion The expression of Cx43 in hippocampus was closely related to the pathogenic mechanism of epilepsy. VNS de-creased the expression of Cx43 ih hippocampus and inhibited the epileptic seizure.

关 键 词：迷走神经刺激 海马 星形胶质 缝隙连接蛋白43 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]