乏氧对人牙髓细胞增殖能力及碱性磷酸酶活性的影响  

Effects of hypoxia on the proliferation and alkaline phosphatase activity of human dental pulp cells in vitro

作　　者：唐开亮[1] 杜毅[1] 于西佼[1] 

机构地区：[1]济南市口腔医院牙体牙髓科,山东济南250001

出　　处：《牙体牙髓牙周病学杂志》2013年第11期681-684,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:探讨乏氧环境对人牙髓细胞(hDPCs)增殖能力及碱性磷酸酶(alkaline phosphatase activity,ALP)活性的影响。方法:取第4代hDPCs分别在氧浓度为200mL/L(对照组)和10mL/L(乏氧组)的环境下进行培养;分别于培养后24h和48h,采用MTT法和PNPP法检测各组各时间点牙髓细胞的增殖能力及其ALP。结果:无论是乏氧组还是对照组,培养48h时的细胞增殖能力均明显高于其24h的增殖能力(P<0.05);而且同一时间点内乏氧组的增殖能力高于对照组(P<0.05)。两组中培养48h的ALP活性均明显低于其培养24h(P<0.05);但同一时间点内乏氧组的ALP活性明显高于对照组(P<0.05)。结论:乏氧环境能增强人牙髓细胞的增殖能力及碱性磷酸酶活性。AIM： To investigate the effects of hypoxia on the proliferation and alkaline phosphatase activity of human dental pulp cells （ hDPCs ）. METHODS ： hDPCs of passage 4 were incubated in normoxie condition （ 200 mL/L 02 ） and hypoxic condition （ 10 mL/L 02 ） for 24 h and 48 h trspevtively. Cell proliferation was determined by MTT assay. Alkaline phosphatase （ALP） activity was measured by PNPP method. RESULTS： Cell proliferation rates and ALP activity were significantly enhanced in hypoxia group as compared with those of normoxic group （ P 〈 0.05 ）. CONCLUSION ： Hypoxia can promote the proliferation and ALP activity of hDPCs.

关 键 词：牙髓细胞 乏氧 增殖 碱性磷酸酶 

分 类 号：R780.2[医药卫生—口腔医学]