尿苷二磷酸葡萄糖促进未成熟脑白质内在修复潜能的体内研究  

Effect of application of uridine diphosphate-glucose on self-repairment potentiality of immature cerebral white matter in vivo

作　　者：毛凤霞[1] 陈惠金[1] 钱龙华[1] 李文娟[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所,上海200092

出　　处：《临床儿科杂志》2013年第11期1059-1065,共7页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.81070989)

摘　　要：目的探讨体内应用尿苷二磷酸葡萄糖(UDP糖)对未成熟脑白质内在修复潜能的促进作用。方法 5日龄大鼠随机分为Sham组、脑室周围白质软化(PVL)组和UDP组,Sham组为假手术组,PVL组和UDP组建立PVL模型,UDP组缺氧后即刻经腹腔一次性注射UDP糖2000mg/kg。应用免疫荧光三标技术检测体内新生祖细胞的增殖和分化情况,末端转移酶标记技术(TUNEL法)观察脑白质细胞的凋亡情况,并分别于建模后7 d及21 d进行光、电镜脑白质病理和髓鞘形成情况评估。结果 UDP组白质新生神经元-神经胶质2型抗原(NG2+)阳性祖细胞数、新生少突胶质细胞前体标记物(O4+)阳性、少突胶质细胞(OLs)前体、环核苷磷酸二酯酶(CNPase+)阳性、不成熟OL和髓鞘碱性蛋白(MBP+)阳性、成熟OL数均多于同时段PVL组,差异有统计学意义(P均<0.05);白质凋亡细胞数、光镜下病理改变、白质髓鞘形成个数及髓鞘厚度均优于PVL组,差异有统计学意义(P均<0.05)。结论体内应用UDP糖能明显促进白质胶质祖细胞的激活、增殖及进一步分化和成熟,并能明显降低白质新生细胞凋亡率,改善脑白质病变和髓鞘形成。Objectives To explore the effect of application of uridine diphosphate-glucose （UDP-glucose） on self-repairment potentiality of immature white matter （WM） in vivo. Methods Five-day-old rats were randomly divided into sham, periventricular leukomalacia （PVL） and UDP-glucose groups. The PVL model was constructed in the PVL and UDP groups, and UDP-glucose （2000mg/kg） was induced by an intraperitoneal injection at once to the rats of UDP group. PVL in-duced proliferation and differentiation of WM-glial progenitor cells invivo were detected by using the three-label lfuorescent immunoanalysis, the apopotosis in WM cell was observed by TUNEL test, and the pathology of WM and myelination were evaluated by light and electron microscopy at day 7 and day 21 after PVL model construction. Results The numbers of new WM-progenitors （NG2＋）, oligodendrocytes （OLs） progenitor marker （O4＋）, OL precursors, cyclic nucleotide phosphodiesterase （CNPase＋）, immature OLs and myelin basic protein （MBP＋）, and mature OLs in the UDP-glucose group are signiifcantly grea-ter than those in the PVL group at each time interval after induction of PVL （P〈0.05）. The numbers of the apoptotic cells in UDP-glucose group are less than those in the PVL groups. Under light and electron microscopy, the white matter pathological changes and myelination were found to be better than those in the PVL group （P〈0.05）. Conclusion The application of UDP-glucose can induce the WM-progenitors to activate, proliferate and differentiate into immature and mature OLs. UDP-glucose can also signiifcantly reduce the apoptotic rate of the WM-new glia cells;improve the white matter pathological changes and the myelin formation.

关 键 词：脑室周围白质软化 脑白质 尿苷二磷酸葡萄糖 少突胶质细胞 大鼠 

分 类 号：R363[医药卫生—病理学]