双轴旋转运动刺激对大鼠前边缘皮质神经元放电的影响  

Effects of double axes rotation on neuronal firing of prelimbic cortex of rat

作　　者：冯斑[1] 付强[2] 黄汾生[1] 张富兴[1] 李金莲[1] 李云庆[1] 

机构地区：[1]第四军医大学人体解剖与组织胚胎学教研室暨梁銶琚脑研究中心,西安710032 [2]西安市公安局霸桥分局刑警大队,西安710038

出　　处：《神经解剖学杂志》2013年第6期615-619,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81071105;81171279)

摘　　要：目的：探索前庭信息是否调节前边缘皮质（prelimbic cortex，PrL）区神经元的活动。方法：体重130—150g雄性sD大鼠，分成两组：对照组和运动刺激组。运动刺激组动物接受2h双轴旋转运动刺激，以刺激前庭感受器。采用全细胞脑片膜片钳技术，分别在电流钳和电压钳模式下，刺激PrL浅层（Ⅱ／Ⅲ层）并记录PrL深层（Ⅴ／Ⅵ层）神经元，分析动作电位（action potential，AP）特性和诱发的兴奋性突触后电流（evoked excitatory postsynaptic current，eEPSC）。结果：2h双轴旋转刺激没有改变PrL神经元AP阈值、幅值、半幅时程、峰值上升相最大斜率以及峰值下降相最大斜率等参数（P〉0．05，n=10）；但向神经元输入波宽180ms，80～280pA的脉冲电流后，旋转刺激组PrL神经元放电数目显著增加（P〈0．05，n=8）。结论：前庭信息可影响PrL神经元活动，故PrL很可能是接受和处理前庭信息的高级脑结构之一。Objective： To investigate whether vestibular signals modulate the neuronal activities in prelimbic cortex （PrL） of rat. Methods： Male Sprague-Dawley rats, 130- 150 g, were used and divided into two groups： control and motion-stimulated groups, and animals in the later group were subjected to double-axes rotation for 2 hours which stimula- ted vestibular end organs. In whole cell patch mode on brain slices, superfacial layers （ layers Ⅱ and Ⅲ） were stimulated and neurons in deep layers （layers Ⅴ and Ⅵ） were recorded. Properties of action potentials （AP） and evoked excitatory postsynaptic currents （eEPSCs） were obtained and analyzed. Results： Two-hour doube-axes rotation did not alter the fol- lowing AP-related parameters ： AP threshold, amplitude, half-width, maximum rise slope and maximum dacay slope （P 〉 0.05, n = 10） ; however, intracellular current pulses ranging from 80 -280 pA with duration of 180 ms evoked significant difference in the number of spike firing in the cases where anminals received 2 h double-axes rotation （P 〈 0.05, n = 8）. Conclusion： Vestibular signals exert modulatory action on PrL neurons and PrL is likely one of the higher cortical areas that receive and process vestibular signals.

关 键 词：前庭器官 扣带回 前边缘皮质 动作电位 膜片钳 大鼠 

分 类 号：R338[医药卫生—人体生理学]