新型多重PCR方法及其在呼吸道病毒诊断上的应用  

The Novel Multiplex PCRs and Their Applications in Respiratory Viruses Diagnosis

作　　者：李瑾[1,2] 申红卫[3] 秦萌[4] 马学军[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京市102206 [2]中国疾病预防控制中心营养与食品安全所,北京市100050 [3]深圳市福田区疾病预防控制中心深圳市518040 [4]北京市丰台区疾病预防控制中心,北京市100071

出　　处：《病毒学报》2013年第6期638-645,共8页Chinese Journal of Virology

基　　金：传染病防治重大专项(2011ZX10004-001,2012ZX10004-215,2013ZX10004-202)

摘　　要：急性呼吸道感染广泛分布于人群中,主要由呼吸道病毒引起,在老年人和婴幼儿中具有较高的发病率和死亡率,给社会带来严重的经济负担。快速、准确检测出病原体对临床早期诊断和指导治疗有重要意义。多重聚合酶链式反应(PCR)技术具有PCR的高灵敏度和快速检测的特点,还能实现多病原的高通量检测。基于多重PCR技术开发的商业化试剂盒能同时检测12种以上的呼吸道病毒,能达到与实时荧光定量PCR(real-time PCR)相当的灵敏度和特异性,为呼吸道病毒的检测与分型提供了新的选择。本文综合了近年来的发展成果,对新型多重PCR方法的原理及其在呼吸道病毒诊断上的应用进行了综述。Acute respiratory tract infections （ARTIs） are widely distributed among the population, mainly caused by respiratory viruses. ARTIs are responsible for significant morbidity and mortality among the elderly and infants or young children, causing a serious economic burden. The rapid and accurate identifi- cation of a pathogen will provide a guideline for the clinical diagnosis and therapy. Multiplex polymerase chain reaction （PCR） technologies combine the rapidness and high sensitivity of PCR with high through- put, thus achieving the capability of detecting multiple pathogens simultaneously. The commercial kits based on these multiplex PCR methods allow to detect more than twelve respiratory viruses simultaneous- ly, reaching the comparable sensitivities and specificities to those of real-time PCR. The recent progress of novel multiplex PCR assays and their principles as well as applications in respiratory virus diagnosis were reviewed in this paper.

关 键 词：多重PCR 呼吸道病毒 诊断 

分 类 号：R373.9[医药卫生—病原生物学]