NLRP3在大鼠实验性根尖周炎中的表达  

Expression of NLRP3 in Induced Rat Periapical Lesions

作　　者：刘生波[1] 刘元元[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2013年第11期1002-1004,共3页Journal of Oral Science Research

基　　金：国家自然科学青年科学基金项目(81102228)

摘　　要：目的:了解NLRP3在大鼠实验性根尖周炎发生发展中的作用。方法:30只大鼠双侧下颌第一磨牙开髓后暴露于口腔环境中,分别于开髓后0、1、7、14d和21d处死大鼠,分离双侧下颌骨。组织学处理后HE染色观察根尖周组织炎症状况,免疫组化检测NLRP3在根尖周组织的表达及定位。结果:炎性根尖周组织成纤维细胞及多种炎症细胞均有NLRP3阳性表达,且表达的数量和根尖周组织的炎症浸润强度显著正相关(P<0.01)。结论:NLRP3在根尖周组织中表达并在根尖周炎的发生发展中起着重要作用。Objective： To investigate the role of NLRP3 in the development of experimental periapical lesions. Methods： Periapical lesions developed within 21 days after pulp exposure of mandibular first molar in SD rats. The rats were randomly sacrificed at 0, 1, 7, 14 and 21 days after pulpal exposure. The bilateral mandibles were extrac- ted for histological processing, then they were hematoxylin--eosin （HE） stained to examine inflammation intensity in the apical area and immunohistochemiscally examined for the expression and localization of NLRP3. Results： In the inflammatory periapical tissues, NLRP3 can be detected in the cytoplasm of fibroblast, neutrophils, monocytes, macrophages and vascular endothelial cells. And the number of NLRP3--positive cells was significantly correlated with the intensity of inflammatory infiltration （P〈0.01）. Conclusion： NLRP3 is expressed in the inflammatory periapical tissues, and plays an important role in the development of periapical lesions. E

关 键 词：NLRP3先 天性免疫 根尖周炎 

分 类 号：R781.4[医药卫生—口腔医学]