基于两个相关群体的玉米籽粒类黄酮含量的QTL定位  

QTL Analysis of Flavonoid Content in Maize(Zea mays L.) Kernel Using Two Connected Populations

作　　者：孙海艳[1] 覃鸿妮[2] 蔡一林[3] 王久光[3] 王国强[3] 吕彦达 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]苏州工业园区服务外包职业学院,苏州215123 [3]西南大学玉米研究所,重庆400716

出　　处：《农业生物技术学报》2013年第11期1313-1320,共8页Journal of Agricultural Biotechnology

基　　金：重庆市重大科技专项(No.CSTC2010AA1022);中央高校基本科研业务费专项(No.XDJK2009C122)

摘　　要：类黄酮作为一种多酚类次生代谢产物在玉米籽粒中具有天然的抗氧化活性，对其进行数量性状位点（quantitative trait locus, QTL）分析，对玉米（Zea mays L.） 种皮色泽相关基因的定位和克隆具有重要的理论意义。本研究利用西南地区常用玉米自交系木6（Mu6）和玉米骨干自交系Mo17为母本，全黑SDM自交系为父本分别杂交构建的2个F2：3家系为定位群体，对重庆和云南2个环境下的籽粒类黄酮含量进行QTL分析。结果表明，在2个群体中共检测出8个QTL，分布于第1、4、7、9和10染色体上，单个QTL可解释的表型变异为5.27%~13.76%；在2个环境中检测到1个一致性QTL与同一标记umc2332连锁，位于bin7.04处，该区域内的QTL贡献率均在10%以上。研究表明类黄酮含量表现为多基因控制的数量性状，与这些QTL紧密相关的分子标记可用于玉米主要色素的分子标记辅助选择。Flavonoids are polyphenolic secondary metabolites, existing in maize（Zea mays L.）. They possess natural antioxidant. It is important to comprehensively study the QTL mapping for fine mapping and positional cloning of pigmentation-related genes. In this paper, two connected F2：3 populations derived from crosses of Mu6×SDM and Mo17×SDM were used to identify QTLs for flavonoid content in maize kernels.The population was grown at Chongqing and Yunnan. A total of 8 QTLs were detected on two related populations. Eight QTLs were located on chromosome 1, 4, 7, 9 and 10, respectively explaining 5.27%~13.76% of phenotypic variation. One QTL located on chromosome 7 was linked with the same marker umc2332 at bin7.04 in two environments, and the QTL explained above 10% of phenotypic variation. These results suggest that the flavonoid content are controlled with a pattern of quantitative trait inheritance, and molecular markers closely linked with these QTLs can be applied in marker-assisted selection of pigment components in maize.

关 键 词：玉米(Zea mays L ) 类黄酮 数量性状位点(QTL) 

分 类 号：S511[农业科学—作物学]