朗德鹅肝细胞核因子4α基因(HNF4α)cDNA克隆、组织表达及填饲对其表达量的影响  

Cloning and Tissue Expression of Hepatocyte Nuclear Factor 4α Gene(HNF4α) in Landes Geese(Anser anser) and Its Responding to Overfeeding

作　　者：蒋拾贝[1] 孙晓先[1] 牛俊伟[1] 段修军[2] 董飚[2] 龚道清[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏农牧科技职业学院,泰州225300

出　　处：《农业生物技术学报》2013年第11期1365-1372,共8页Journal of Agricultural Biotechnology

基　　金：江苏省科技支撑计划项目(No.BE2011328)

摘　　要：肝细胞核因子4α(hepatic nuclear factor 4 alpha,HNF4α)属于细胞核受体超家族成员,在肝脏发育、肝细胞分化成熟过程中起重要调控作用。为了探讨HNF4α基因在朗德鹅(Anser anser)肥肝形成过程中的作用,本研究扩增了朗德鹅HNF4α基因序列,获得该基因的全长cDNA,并用实时定量PCR技术检测该基因在朗德鹅心脏、肝脏、脾脏、肺脏、肾脏、胸肌、腿肌和腹脂8个组织中的表达情况及填饲对其在肝脏中表达量的影响。研究结果显示,朗德鹅HNF4α基因序列长为1 371 bp,编码456个氨基酸,与鸡氨基酸序列同源性为99.12%;组织表达检测结果显示,该基因主要在肝脏和肾脏中表达,在其他组织中表达量很低;填饲组该基因在肝脏中的表达量约是对照组表达量的1/2,且差异显著(P<0.05)。本研究结果表明,HNF4α基因可能在肝脏脂质代谢基因表达调控网络中起重要作用,为进一步研究HNF4α在鹅肥肝形成中的功能提供参考依据。Hepatocyte nuclear factor 4α （HNF4α） is a member of the nuclear receptor superfamily, it plays an crucial role in liver organogenesis and hepatocyte differentiation. In order to investigate the role of HNF4α in the formation of goose fatty liver, we amplified the goose（Anser anser） HNF4α gene full-length cDNA sequence, and detected the expression levels in 8 tissues（heart, liver, spleen, lung, kidney, pectorale, crureus and abdominal fat） as well as the effect of overfeeding on HNF4α gene in liver by qRT-PCR. The results showed that the cDNA sequence of HNF4α gene was 1 371 bp and encoded 456 amino acids, and the amino acids had high homology with chicken（99.12%）. In addition, HNF4α gene was mainly expressed in liver and kidney of goose, but it had lower expression level in other tissues. After overfeeding, the expression of HNF4α gene in liver was significantly decreased compared with the control group（P〈0.05）. The results indicated that the HNF4α gene might play an important role in lipid metabolism. It provides the basic dates for the further study that analysis the function of HNF4α gene during formation of geese fatty liver.

关 键 词：肝细胞核因子4Α 肥肝 基因克隆 MRNA表达 

分 类 号：S835[农业科学—畜牧学]