孕酮对脑损伤后环氧化酶-2表达和脑水肿的影响  

Effects of progesterone treatment on expression of COX- 2 and brain edema after traumatic brain injury

作　　者：司道文[1] 杨鹏[1] 郭艳华[2] 孙敬宇[2] 赵金芝[2] 罗晓燕[3] 程勇[4] 栗占营[3] 李云[1] 

机构地区：[1]河北联合大学基础医学院,河北唐山063000 [2]唐山市丰润区人民医院 [3]开滦总医院 [4]河北大学附属医院

出　　处：《河北联合大学学报（医学版）》2013年第6期759-760,共2页Journal of North China Coal Medical College

基　　金：河北省教育厅科学研究计划项目(编号:Z2011168);唐山市科学技术研究与发展计划项目(编号:12140209A-55)

摘　　要：①目的观察孕酮对大鼠脑损伤后环氧化酶-2表达和脑水肿的影响。②方法雄性SD大鼠随机分为假手术组、脑损伤组、安慰剂组和孕酮治疗组。按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型。各组于伤后1、24、72小时取材。用免疫组织化学法观察大鼠皮质COX-2的表达水平变化,并测量脑组织含水量。③结果脑损伤组和安慰剂组大鼠皮质COX-2阳性细胞数和脑水肿较假手术组显著增加;孕酮治疗组与脑损伤组和安慰剂组比较,大鼠皮质COX-2阳性细胞数和脑水肿明显减少,差异有统计学意义(P<0.05)。④结论孕酮可能通过下调COX-2的表达,降低脑内炎症反应,进而降低脑水肿,发挥脑损伤保护作用。Objective To investigate effects of progesterone treatment on COX -2 expression and brain edema after traumat- ic brain injury in rats. Methods Male Spraque - Dawley rats were randomly divided sham - operated group, TBI group, Vehicle and PROG -treated group. The rat model of TBI was duplicated with the improved Feeney~ method. The rats were killed respective- ly at 1,24, 72 after injury. The changes of the positive cell numbers of COX - 2 were examined by immunohistochemistry and brain edema were measured. Results The positive cell numbers of COX - 2 and brain edema of the TBI and Vehicle group were in- creased. The positive cell numbers of COX - 2 and brain edema of the PROG - treated group were distinctly less than those of the TBI and Vehicle group（ P 〈 0.05）. Conclusion The protective effect of progesterone on brain edema may be induced by down - reb-ulation of COX -2 expression and reduce inflammation after traumatic brain injury.

关 键 词：孕酮 脑损伤 环氧化酶-2 脑水肿 

分 类 号：R362[医药卫生—病理学]